Bråve Andreas, Johansen Kari, Palma Paolo, Benthin Reinhold, Hinkula Jorma
Swedish Institute for Infectious Disease Control & Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Solna, Sweden.
Vaccine. 2008 Nov 5;26(47):5957-66. doi: 10.1016/j.vaccine.2008.08.060. Epub 2008 Sep 16.
This study was designed to determine the impact of maternal HIV-1 specific immunity on HIV-DNA immunization of 2-week-old pups during the breast-feeding period. Adult female mice received intranasal or intradermal HIV-DNA (gp160Env, p37Gag, Nef, Tat and Rev) prime and recombinant protein boost immunizations, which induced mucosal and systemic HIV-1 specific B and T cell responses. Intranasal administration of the immunogens induced higher serum IgG titers to HIV antigens than intradermal immunization. Furthermore, high HIV-1 specific fecal IgA titers were obtained in mice immunized by intranasal administration. The capacity to respond to the same immunogens (one single prime with DNA and one boost with recombinant protein) was then compared in pups born to mothers with HIV-1-specific immune responses and pups born to non-vaccinated mothers. Immune responses to the largest number of antigens were detected in pups born to mothers with the highest HIV-1-specific immune responses. These data show that HIV-1 DNA-plasmid immunization during breast-feeding and recombinant protein boosting shortly thereafter enhance the breadth of humoral HIV-1-specific immune responses.
本研究旨在确定母体HIV-1特异性免疫对哺乳期2周龄幼崽HIV-DNA免疫的影响。成年雌性小鼠接受鼻内或皮内HIV-DNA（gp160Env、p37Gag、Nef、Tat和Rev）初免及重组蛋白加强免疫，诱导黏膜和全身HIV-1特异性B细胞和T细胞反应。与皮内免疫相比，鼻内给予免疫原诱导的HIV抗原血清IgG滴度更高。此外，通过鼻内给药免疫的小鼠获得了高HIV-1特异性粪便IgA滴度。然后比较了具有HIV-1特异性免疫反应的母亲所生幼崽和未接种疫苗母亲所生幼崽对相同免疫原（一次DNA初免和一次重组蛋白加强）的反应能力。在HIV-1特异性免疫反应最强的母亲所生幼崽中检测到对最多抗原的免疫反应。这些数据表明，哺乳期进行HIV-1 DNA质粒免疫并在此后不久进行重组蛋白加强免疫可增强体液HIV-1特异性免疫反应的广度。
