Monteil-Rivera Fanny, Halasz Annamaria, Manno Dominic, Kuperman Roman G, Thiboutot Sonia, Ampleman Guy, Hawari Jalal
Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montréal, Québec, Canada H4P 2R2.
Environ Pollut. 2009 Jan;157(1):77-85. doi: 10.1016/j.envpol.2008.07.030. Epub 2008 Sep 17.
Hexanitrohexaazaisowurtzitane (CL-20) is an emerging explosive that may replace the currently used explosives such as RDX and HMX, but little is known about its fate in soil. The present study was conducted to determine degradation products of CL-20 in two sandy soils under abiotic and biotic anaerobic conditions. Biotic degradation was prevalent in the slightly acidic VT soil, which contained a greater organic C content, while the slightly alkaline SAC soil favored hydrolysis. CL-20 degradation was accompanied by the formation of formate, glyoxal, nitrite, ammonium, and nitrous oxide. Biotic degradation of CL-20 occurred through the formation of its denitrohydrogenated derivative (m/z 393 Da) while hydrolysis occurred through the formation of a ring cleavage product (m/z 156 Da) that was tentatively identified as CH(2)=N-C(=N-NO(2))-CH=N-CHO or its isomer N(NO(2))=CH-CH=N-CO-CH=NH. Due to their chemical specificity, these two intermediates may be considered as markers of in situ attenuation of CL-20 in soil.
六硝基六氮杂异伍兹烷（CL - 20）是一种新兴炸药，可能会取代目前使用的炸药，如黑索今（RDX）和奥克托今（HMX），但人们对其在土壤中的归宿知之甚少。本研究旨在确定CL - 20在两种砂质土壤中在非生物和生物厌氧条件下的降解产物。生物降解在有机碳含量较高的微酸性VT土壤中普遍存在，而微碱性的SAC土壤则有利于水解。CL - 20的降解伴随着甲酸盐、乙二醛、亚硝酸盐、铵和一氧化二氮的形成。CL - 20的生物降解是通过形成其脱硝基氢化衍生物（m/z 393 Da）发生的，而水解是通过形成一种环裂解产物（m/z 156 Da）发生的，该产物暂定为CH(2)=N-C(=N-NO(2))-CH=N-CHO或其异构体N(NO(2))=CH-CH=N-CO-CH=NH。由于它们的化学特异性，这两种中间体可被视为土壤中CL - 20原位衰减的标志物。
