Lian D X, Yang L, Yun W X, Hua S, Hu C
Shandong Academy of Medical Science, Jinan, China.
J Chromatogr. 1991 Apr 12;542(2):526-30. doi: 10.1016/s0021-9673(01)88788-4.
A method is described for the determination of aldicarb and its metabolites (the sulphoxide and sulphone) in urine by gas chromatography with flame photometric detection (GC-FPD). The sample was concentrated with a column containing activated charcoal and Florisil, and then eluted with dichloromethane-acetone (1:1, v/v). The aldicarb and aldicarb sulphoxide in the eluate solution were oxidized to aldicarb sulphone and the total sulphone concentration was determined by GC-FPD after extraction with dichloromethane and clean-up with an activated charcoal column. The detection limit was 0.0024 mg/l. The mean recoveries from spiked urine in the range 0.04-0.12 mg/l were 90.9%, 86.6%, 92.6% for aldicarb, aldicarb sulphoxide and aldicarb sulphone, respectively.
描述了一种采用带火焰光度检测的气相色谱法（GC-FPD）测定尿液中涕灭威及其代谢物（亚砜和砜）的方法。样品用装有活性炭和弗罗里硅土的柱进行浓缩，然后用二氯甲烷 - 丙酮（1:1，v/v）洗脱。洗脱液中的涕灭威和涕灭威亚砜被氧化为涕灭威砜，用二氯甲烷萃取并用活性炭柱净化后，通过GC-FPD测定总砜浓度。检测限为0.0024 mg/l。在0.04 - 0.12 mg/l范围内加标尿液中涕灭威、涕灭威亚砜和涕灭威砜的平均回收率分别为90.9%、86.6%、92.6%。
