Shen Hong, Wan Yuping, Wu Xiaosheng, Zhang Yu, Li Jingwen, Cui Tingting, Sun Han, Cui Haifeng, He Kailun, Hui Guangpeng, Chen Xu, Liu Guoqiang, Du Meihong
Biological Inspection Department, Zhejiang Institute for Food and Drug Control, Hangzhou, China.
Beijing Kwinbon Biotechnology Co., Ltd., Beijing, China.
Front Nutr. 2022 Aug 23;9:976284. doi: 10.3389/fnut.2022.976284. eCollection 2022.
The common carbamate insecticide aldicarb is considered one of the most acutely toxic pesticides. Herein, rational design was used to synthesize two haptens with spacers of different carbon chain lengths. The haptens were then used to immunize mice. The antibodies obtained were evaluated systematically, and a colloidal gold immunochromatographic strip was developed based on an anti-aldicarb monoclonal antibody. The 50% inhibition concentration and linear range of anti-aldicarb monoclonal antibody immunized with Hapten 1 were 0.432 ng/mL and 0.106-1.757 ng/mL, respectively. The cross-reactivities for analogs of aldicarb were all <1%. The limit of detection of the colloidal gold immunochromatographic strip was 30 μg/kg, and the average recoveries of aldicarb ranged from 80.4 to 110.5% in spiked samples. In the analysis of spiked samples, the test strip could accurately identify positive samples detected by the instrumental method in the GB 23200.112-2018 standard but produced some false positives for negative samples. This assay provides a rapid and accurate preliminary screening method for the determination of aldicarb in agricultural products and environments.
常见的氨基甲酸酯类杀虫剂涕灭威被认为是毒性最强的农药之一。在此，采用合理设计合成了两种具有不同碳链长度间隔基的半抗原。然后用这些半抗原免疫小鼠。对获得的抗体进行了系统评估，并基于抗涕灭威单克隆抗体制备了胶体金免疫层析试纸条。用半抗原1免疫的抗涕灭威单克隆抗体的50%抑制浓度和线性范围分别为0.432 ng/mL和0.106 - 1.757 ng/mL。涕灭威类似物的交叉反应率均<1%。胶体金免疫层析试纸条的检测限为30 μg/kg，在加标样品中涕灭威的平均回收率为80.4%至110.5%。在加标样品分析中，该试纸条能准确识别GB 23200.112 - 2018标准中仪器法检测出的阳性样品，但对阴性样品会产生一些假阳性结果。该方法为农产品和环境中涕灭威的测定提供了一种快速准确的初步筛选方法。
