Casado Javier G, Delgado Elena, Patsavoudi Evangelia, Durán Esther, Sanchez-Correa Beatriz, Morgado Sara, Solana Rafael, Tarazona Raquel
Department of Physiology, Immunology Unit, Faculty of Veterinary Science, University of Extremadura, Caceres, Spain.
Tumour Biol. 2008;29(5):304-10. doi: 10.1159/000156707. Epub 2008 Sep 19.
HNK-1 epitope, also known as CD57, is expressed on a wide range of cells and has been related to cell to cell and cell to extracellular matrix interactions. Expression of the HNK-1 epitope has been considered a prognostic factor in several tumours, as it has been associated with the risk of metastasis. HNK-1 has been found to be expressed on uveal and cutaneous melanoma and proposed as a useful marker of the risk of metastasis. We have analysed the HNK-1 expression on a large panel of melanoma cell lines, the involvement of this epitope in melanoma cell adhesion, as well as its migrative and invasive behaviour. HNK-1 was highly expressed in 12.9% of melanoma cell lines, and in vitro experiments using invasive melanoma cell lines demonstrated that an HNK-1 blockade reduces cell adhesion to extracellular matrix as well as their migrative and invasive ability. These data support the functional relevance of HNK-1 expression in metastatic melanoma.
HNK-1表位，也称为CD57，在多种细胞上表达，并且与细胞间以及细胞与细胞外基质的相互作用有关。HNK-1表位的表达在几种肿瘤中被认为是一种预后因素，因为它与转移风险相关。已发现HNK-1在葡萄膜和皮肤黑色素瘤上表达，并被提议作为转移风险的有用标志物。我们分析了大量黑色素瘤细胞系上的HNK-1表达、该表位在黑色素瘤细胞黏附中的作用及其迁移和侵袭行为。HNK-1在12.9%的黑色素瘤细胞系中高表达，使用侵袭性黑色素瘤细胞系的体外实验表明，HNK-1阻断可降低细胞对细胞外基质的黏附以及它们的迁移和侵袭能力。这些数据支持了HNK-1表达在转移性黑色素瘤中的功能相关性。
