Tsukimori Kiyomi, Yoshitomi Tomoyuki, Morokuma Seiichi, Fukushima Kotaro, Wake Norio
Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Am J Hypertens. 2008 Dec;21(12):1343-6. doi: 10.1038/ajh.2008.289. Epub 2008 Sep 18.
Increased serum uric acid has been postulated to directly promote oxidative stress. The aim of this study was to investigate whether serum uric acid level correlated with superoxide generation and oxidative stress in preeclampsia.
We assessed serum uric acid levels, plasma hydrogen peroxide levels, and plasma protein carbonyl levels in normal pregnant (n = 14) and preeclamptic women (n = 17). Serum uric acid and plasma hydrogen peroxide levels were determined by the uricase-peroxidase method, a combined enzymatic-colorimetric method. Plasma carbonyl levels were measured by ELISA.
The mean serum uric acid values were significantly elevated in preeclampsia (6.6 +/- 1.5 mg/dl) compared with normal pregnancy (4.0 +/- 0.7 mg/dl) (P < 0.001). Plasma protein carbonyl levels were also significantly elevated in preeclampsia (7.72 +/- 4.10 nmol/mg) compared with normal pregnancy (2.85 +/- 3.06 nmol/mg) (P = 0.0011). In addition, the plasma hydrogen peroxide levels were significantly higher in preeclampsia (66.9 +/- 10.9 micromol/l) compared with normal pregnancy (50.1 +/- 5.6 micromol/l) (P < 0.001). Serum uric acid levels correlated significantly and positively with plasma hydrogen peroxide levels (r(2) = 0.2965, P = 0.0027) and plasma protein carbonyl levels (r(2) = 0.2011, P = 0.0129).
The increased serum uric acid levels correlated closely with plasma hydrogen peroxide levels and plasma protein carbonyl levels in preeclampsia. Therefore, the serum uric acid level serves as an indicator of the underlying oxidative stress in preeclampsia.
血清尿酸升高被认为可直接促进氧化应激。本研究旨在探讨血清尿酸水平与子痫前期中超氧化物生成及氧化应激是否相关。
我们评估了正常孕妇（n = 14）和子痫前期孕妇（n = 17）的血清尿酸水平、血浆过氧化氢水平及血浆蛋白羰基水平。血清尿酸和血浆过氧化氢水平采用尿酸酶 - 过氧化物酶法（一种联合酶比色法）测定。血浆羰基水平通过酶联免疫吸附测定法（ELISA）测量。
与正常妊娠（4.0±0.7mg/dl）相比，子痫前期患者的平均血清尿酸值显著升高（6.6±1.5mg/dl）（P < 0.001）。与正常妊娠（2.85±3.06nmol/mg）相比，子痫前期患者的血浆蛋白羰基水平也显著升高（7.72±4.10nmol/mg）（P = 0.0011）。此外，与正常妊娠（50.1±5.6μmol/l）相比，子痫前期患者的血浆过氧化氢水平显著更高（66.9±10.9μmol/l）（P < 0.001）。血清尿酸水平与血浆过氧化氢水平显著正相关（r² = 0.2965，P = 0.0027），与血浆蛋白羰基水平也显著正相关（r² = 0.2011，P = 0.0129）。
子痫前期患者血清尿酸水平升高与血浆过氧化氢水平及血浆蛋白羰基水平密切相关。因此，血清尿酸水平可作为子痫前期潜在氧化应激的一个指标。
