Harma Mehmet, Harma Muge, Erel Ozcan
Departments of Obstetrics and Gynecology, Medical School, University of Harran, Sanliurfa, Turkey.
Eur J Obstet Gynecol Reprod Biol. 2005 Jan 10;118(1):47-51. doi: 10.1016/j.ejogrb.2004.04.012.
Preeclampsia is one of the most serious complications of pregnancy. Free radical damage has been implicated in the pathophysiology of this condition. In this study, we aimed to measure the antioxidant capacity in plasma samples from normotensive and preeclamptic pregnant women to evaluate their antioxidant status using a more recently developed automated measurement method.
Our study group contained 42 women, 24 of whom had preeclampsia, while 18 had normotensive pregnancies. We measured the total plasma antioxidant capacity for all patients, as well as the levels of four major individual plasma antioxidant components; albumin, uric acid, ascorbic acid and bilirubin, and as a reciprocal measure, their total plasma peroxide levels.
Statistically significant differences (determined using Student's t-test) were noted between the normotensive and the preeclamptic groups for their total antioxidant responses and their vitamin C levels (1.31 +/- 0.12 mmol versus 1.06 +/- 0.41 mmol Trolox eq./L; 30.2 +/- 17.83 micromol/L versus 18.1 +/- 11.37 micromol/L, respectively), which were both considerably reduced in the preeclamptic patients. In contrast, the total plasma peroxide levels were significantly elevated in this group (49.8 +/- 14.3 micromol/L versus 38.8 +/- 9.6 micromol/L).
We found a decreased total antioxidant response in preeclamptic patients using a simple, rapid and reliable automated colorimetric assay, which may suitable for use in any routine clinical biochemistry laboratory, and considerably facilitates the assessment of this useful clinical parameter. We suggest that this novel method may be used as a routine test to evaluate and follow up of the levels of oxidative stress in preeclampsia.
子痫前期是妊娠最严重的并发症之一。自由基损伤与该病症的病理生理学有关。在本研究中，我们旨在使用一种最新开发的自动测量方法，测量正常血压和子痫前期孕妇血浆样本中的抗氧化能力，以评估她们的抗氧化状态。
我们的研究组包含42名女性，其中24名患有子痫前期，18名妊娠血压正常。我们测量了所有患者的血浆总抗氧化能力，以及四种主要的血浆抗氧化成分；白蛋白、尿酸、抗坏血酸和胆红素的水平，作为相应指标，还测量了她们的血浆总过氧化物水平。
正常血压组和子痫前期组在总抗氧化反应和维生素C水平方面存在统计学显著差异（使用学生t检验确定）（分别为1.31±0.12 mmol与1.06±0.41 mmol Trolox当量/升；30.2±17.83微摩尔/升与18.1±11.37微摩尔/升），子痫前期患者的这两项指标均显著降低。相比之下，该组的血浆总过氧化物水平显著升高（49.8±14.3微摩尔/升与38.8±9.6微摩尔/升）。
我们使用一种简单、快速且可靠的自动比色测定法，发现子痫前期患者的总抗氧化反应降低，该方法可能适用于任何常规临床生物化学实验室，并极大地促进了这一有用临床参数的评估。我们建议这种新方法可作为一种常规检测，用于评估和随访子痫前期患者的氧化应激水平。
