用微量量热法测定二甲基亚砜和某些抗生素对培养的人T淋巴瘤细胞的影响。

Effect of dimethyl sulphoxide and some antibiotics on cultured human T-lymphoma cells as measured by microcalorimetry.

作者信息

Lönnbro P, Wadsö I

机构信息

Chemical Centre, University of Lund, Sweden.

出版信息

J Biochem Biophys Methods. 1991 May-Jun;22(4):331-6. doi: 10.1016/0165-022x(91)90039-y.

DOI:10.1016/0165-022x(91)90039-y

PMID:1880336

Abstract

The influence of dimethyl sulphoxide (I), penicillin/streptomycin (II), gentamicin (III), and amphotericin B (IV) on growing human T-lymphoma cells was measured by microcalorimetry. There was a dose-dependent decrease in the heat production rate of the cells after 24 h of incubation with I in concentrations ranging from 0-2% (v/v). At 3.6%, about half of the cells died. II and III had no effect on the cells after incubation for 6 days, at concentrations from 1 to 10 times that of the normal (50-500 IU/ml; 50-500 micrograms/ml). IV was used in combination with II (50 IU/ml; 50 micrograms/ml) and III (50 micrograms/ml), respectively, at concentrations between 0.25 and 7.5 micrograms/ml. After 6 days of incubation, the results were similar to those obtained with II and III separately.

摘要

通过微量量热法测定了二甲基亚砜（I）、青霉素/链霉素（II）、庆大霉素（III）和两性霉素B（IV）对人T淋巴瘤细胞生长的影响。在0-2%（v/v）浓度范围内用I孵育细胞24小时后，细胞的产热速率呈剂量依赖性下降。在3.6%时，约一半的细胞死亡。在浓度为正常浓度的1至10倍（50-500 IU/ml；50-500微克/毫升）下孵育6天后，II和III对细胞没有影响。IV分别与II（50 IU/ml；50微克/毫升）和III（50微克/毫升）联合使用，浓度在0.25至7.5微克/毫升之间。孵育6天后，结果与分别使用II和III时相似。