Bertram J S
Cancer Lett. 1979 Sep;7(5):289-98. doi: 10.1016/s0304-3835(79)80056-7.
The C3H/10T1/2 CL8 cell line is being widely used to study mechanisms of malignant transformation in vitro. As currently employed, the standard assay system uses a combination of penicillin (100 I.U./ml) and streptomycin (50 micrograms/ml) to reduce the occurrence of bacterial contamination. The penicillin component of this mixture has been discovered to cause a reduction in the number of transformed foci which develop after exposure of cells to MCA, DMBA and X-rays. This reduction is dose dependent; 500 I.U./ml virtually eliminates transformation, while 100 I.U./ml causes an approximate 50% decrease in the number of foci. This effect does not appear to be due to overt toxicity and is largely reversible on removal of the antibiotic. Gentamicin (25 micrograms/ml) causes no reduction in the formation of transformed foci when compared to cultures maintained in antibiotic-free medium and offers the advantages of chemical stability, a wider spectrum of antibacterial activity in comparison with penicillin/streptomycin and, in addition, is active against many mycoplasma. It is suggested that future studies with this cell line should ideally be performed without antibiotics or should employ Gentamicin for antibacterial protection.
C3H/10T1/2 CL8细胞系被广泛用于体外研究恶性转化机制。按照目前的使用方法，标准检测系统使用青霉素（100国际单位/毫升）和链霉素（50微克/毫升）的组合来减少细菌污染的发生。已发现该混合物中的青霉素成分会导致细胞暴露于甲基胆蒽、二甲基苯并蒽和X射线后形成的转化灶数量减少。这种减少是剂量依赖性的；500国际单位/毫升几乎消除了转化，而100国际单位/毫升使灶的数量减少了约50%。这种效应似乎不是由于明显的毒性，并且在去除抗生素后在很大程度上是可逆的。与在无抗生素培养基中培养的细胞相比，庆大霉素（25微克/毫升）不会导致转化灶形成减少，并且具有化学稳定性的优点，与青霉素/链霉素相比具有更广泛的抗菌活性，此外，对许多支原体也有活性。建议未来对该细胞系的研究理想情况下应在不使用抗生素的情况下进行，或者应使用庆大霉素进行抗菌保护。
