Ma Minyue, Guo Xuejiang, Wang Fuqiang, Zhao Chun, Liu Zichuan, Shi Zhonghua, Wang Yufeng, Zhang Ping, Zhang Kemei, Wang Ningling, Lin Min, Zhou Zuomin, Liu Jiayin, Li Qingzhang, Wang Liu, Huo Ran, Sha Jiahao, Zhou Qi
Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
J Proteome Res. 2008 Nov;7(11):4821-30. doi: 10.1021/pr800392s. Epub 2008 Sep 20.
The mature oocyte contains the full complement of maternal proteins required for fertilization, the transition to zygotic transcription, and the beginning stages of embryogenesis. Many of these proteins have yet to be characterized. In this study, two-dimensional electrophoresis (2-DE) of mouse metaphase-II (MII) oocyte proteins, stained with silver staining or Pro-Q Diamond dye, was performed to describe the proteome and phosphoproteome of the mouse oocyte derived from ICR mice. A total of 869 selected protein spots, corresponding to 380 unique proteins, were identified successfully by mass spectrometry, in which 90 protein spots representing 53 unique proteins have been stained with Pro-Q Diamond, indicating that they are in phosphorylated forms. All identified proteins were bioinformatically annotated in detail and compared with the embryonic stem cell (ESC) proteome. A proteome reference database for the mouse oocyte was established from the protein data generated in this study, which can be accessed over the Internet ( http://reprod.njmu.edu.cn/2d). This database is the most detailed mouse oocyte proteomic database to date. It should be valuable in expanding our knowledge of the regulation of signaling in oogenesis, fertilization, and embryo development, while revealing potential mechanisms for epigenetic reprogramming.
成熟卵母细胞包含受精、向合子转录过渡以及胚胎发生起始阶段所需的全部母体蛋白质。其中许多蛋白质尚未得到表征。在本研究中，对源自ICR小鼠的小鼠中期II（MII）卵母细胞蛋白质进行了二维电泳（2-DE），并用银染或Pro-Q Diamond染料染色，以描述小鼠卵母细胞的蛋白质组和磷酸蛋白质组。通过质谱成功鉴定出总共869个选定的蛋白质斑点，对应于380种独特蛋白质，其中90个蛋白质斑点代表53种独特蛋白质已被Pro-Q Diamond染色，表明它们处于磷酸化形式。所有鉴定出的蛋白质都进行了详细的生物信息学注释，并与胚胎干细胞（ESC）蛋白质组进行了比较。根据本研究产生的蛋白质数据建立了小鼠卵母细胞蛋白质组参考数据库，可通过互联网访问（http://reprod.njmu.edu.cn/2d）。该数据库是迄今为止最详细的小鼠卵母细胞蛋白质组数据库。它对于扩展我们对卵子发生、受精和胚胎发育中信号调节的认识具有重要价值，同时揭示表观遗传重编程的潜在机制。
