早期胚胎的卵裂由成熟精子中存在的 tRNA 衍生的小 RNA 调控。
Early cleavage of preimplantation embryos is regulated by tRNA-derived small RNAs present in mature spermatozoa.
机构信息
Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China
出版信息
J Biol Chem. 2020 Aug 7;295(32):10885-10900. doi: 10.1074/jbc.RA120.013003. Epub 2020 Jun 2.
tRNA-derived small RNAs (tsRNAs) from spermatozoa could act as acquired epigenetic factors and contribute to offspring phenotypes. However, the roles of specific tsRNAs in early embryo development remain to be elucidated. Here, using pigs as a research model, we probed the tsRNA dynamics during spermatogenesis and sperm maturation and demonstrated the delivery of tsRNAs from semen-derived exosomes to spermatozoa. By microinjection of antisense sequences into fertilized oocytes and subsequent single-cell RNA-seq of embryos, we identified a specific functional tsRNA group (termed here Gln-TTGs) that participate in the early cleavage of porcine preimplantation embryos, probably by regulating cell cycle-associated genes and retrotransposons. We conclude that specific tsRNAs present in mature spermatozoa play significant roles in preimplantation embryo development.
精子来源的 tRNA 衍生的小 RNA（tsRNAs）可作为获得性表观遗传因子，并影响后代表型。然而，特定 tsRNAs 在早期胚胎发育中的作用仍有待阐明。在这里，我们以猪为研究模型，探究了精子发生和精子成熟过程中的 tsRNA 动态变化，并证实了 tsRNAs 可从精液衍生的外泌体递送至精子中。通过将反义序列显微注射到受精卵中，并对胚胎进行单细胞 RNA-seq 分析，我们鉴定出一个特定的功能性 tsRNA 簇（此处称为 Gln-TTGs），其参与猪类囊胚早期的卵裂过程，可能通过调控细胞周期相关基因和逆转座子来实现。综上，我们得出结论，成熟精子中存在的特定 tsRNAs 在胚胎植入前的发育过程中发挥重要作用。
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