Gelberman R H, Steinberg D, Amiel D, Akeson W
Department of Orthopaedic Surgery, Massachusetts General Hospital, Boston 02114.
J Hand Surg Am. 1991 Jul;16(4):686-93. doi: 10.1016/0363-5023(91)90195-h.
Healing canine flexor tendons were treated with early controlled passive mobilization. The repair site and proximal and distal tendon stumps were stained for fibronectin and examined by light microscopy at three, seven, eleven, and seventeen days. Fibronectin increased dramatically in the epitenon adjacent to the repair site seven days after repair, a time when epitenon cellular activity was at its peak. By seventeen days, fibronectin staining had decreased substantially, both at the repair site and in the tendon stumps. A delayed increase in fibronectin activity was noted in the endotenon adjacent to the repair site. Fibronectin production appears to be an important component of the early tendon repair process. Fibroblast chemotaxis and adherence to the substratum in the days after injury and repair appears to be related directly to fibronectin secretion. This study is the first to provide documentation of fibronectin localization in a clinically relevant tendon repair model.
对正在愈合的犬屈肌腱进行早期控制性被动活动。在术后第3天、7天、11天和17天，对修复部位以及近端和远端肌腱残端进行纤连蛋白染色，并通过光学显微镜检查。修复后7天，修复部位附近的腱外表层中纤连蛋白显著增加，此时腱外表层细胞活性达到峰值。到第17天，修复部位和肌腱残端的纤连蛋白染色均大幅减少。在修复部位附近的腱内膜中发现纤连蛋白活性延迟增加。纤连蛋白的产生似乎是早期肌腱修复过程的一个重要组成部分。损伤和修复后数天内，成纤维细胞的趋化性以及对基质的黏附似乎与纤连蛋白的分泌直接相关。本研究首次在临床相关的肌腱修复模型中提供了纤连蛋白定位的文献资料。
