Saumonneau Amélie, Agasse Alice, Bidoyen Marie-Thérèse, Lallemand Magali, Cantereau Anne, Medici Anna, Laloi Maryse, Atanassova Rossitza
Université de Poitiers, FRE CNRS 3091 "Physiologie moléculaire du transport des sucres", Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
FEBS Lett. 2008 Oct 15;582(23-24):3281-7. doi: 10.1016/j.febslet.2008.09.015. Epub 2008 Sep 18.
ASR proteins (abscissic acid, stress, ripening induced) are involved in plant responses to developmental and environmental signals but their biological functions remain to be elucidated. Grape ASR gene (VvMSA) encodes a new transcription factor regulating the expression of a glucose transporter. Here, we provide evidence for some polymorphism of grape ASRs and their identification as chromosomal non-histone proteins. By the yeast two-hybrid approach, a protein partner of VvMSA is isolated and characterized as an APETALA2 domain transcription factor. Interaction of the two proteins is further demonstrated by the BiFC approach and the exclusive nuclear localization of the heterodimer is visualized.
ASR蛋白（脱落酸、胁迫、成熟诱导蛋白）参与植物对发育和环境信号的响应，但其生物学功能仍有待阐明。葡萄ASR基因（VvMSA）编码一种调节葡萄糖转运蛋白表达的新型转录因子。在此，我们提供了葡萄ASR存在某些多态性的证据，并将它们鉴定为染色体非组蛋白。通过酵母双杂交方法，分离出VvMSA的一个蛋白伴侣，并将其鉴定为APETALA2结构域转录因子。通过双分子荧光互补方法进一步证明了这两种蛋白的相互作用，并观察到异二聚体仅定位于细胞核。
