University of Poitiers, UMR CNRS 6503 LACCO, Physiologie Moléculaire du Transport des Sucres chez les Plantes, Bâtiment Botanique B31, 3 rue Jacques Fort, 86022 Poitiers, France.
J Exp Bot. 2012 Feb;63(3):1495-510. doi: 10.1093/jxb/err391. Epub 2011 Dec 3.
Despite the fact that the precise physiological function of ASRs [abscisic acid (ABA), stress, ripening] remains unknown, they have been suggested to play a dual role in the plant response to environmental cues, as highly hydrophilic proteins for direct protection, as well as transcription factors involved in the regulation of gene expression. To investigate further the biological positioning of grape ASR in the hormonal and metabolic signal network, three promoters corresponding to its cDNA were isolated and submited to a detailed in silico and functional analysis. The results obtained provided evidence for the allelic polymorphism of the grape ASR gene, the organ-preferential expression conferred on the GUS reporter gene, and the specific phloem tissue localization revealed by in situ hybridization. The study of glucose and ABA signalling in its transcriptional control, by transfection of grape protoplasts using the dual luciferase system, revealed the complexity of ASR gene expression regulation. A model was proposed allowing a discussion of the place of ASR in the fine tuning of hormonal and metabolic signalling involved in the integration of environmental cues by the plant organism.
尽管 ASR（脱落酸 (ABA)、胁迫、成熟）的确切生理功能尚不清楚，但有人提出它们在植物对环境信号的反应中发挥双重作用，既是高度亲水的蛋白质直接保护剂，又是参与基因表达调控的转录因子。为了进一步研究葡萄 ASR 在激素和代谢信号网络中的生物学定位，分离了与其 cDNA 对应的三个启动子，并进行了详细的计算机分析和功能分析。获得的结果为葡萄 ASR 基因的等位基因多态性、赋予 GUS 报告基因的组织偏好表达以及通过原位杂交揭示的特定韧皮部组织定位提供了证据。通过使用双荧光素酶系统转染葡萄原生质体研究其转录控制中的葡萄糖和 ABA 信号转导，揭示了 ASR 基因表达调控的复杂性。提出了一个模型，允许讨论 ASR 在植物生物体整合环境信号的激素和代谢信号的微调中的位置。
