Varsamis Dimitrios G, Touloupakis Eleftherios, Morlacchi Pietro, Ghanotakis Demetrios F, Giardi Maria Teresa, Cullen David C
Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK.
Talanta. 2008 Oct 19;77(1):42-7. doi: 10.1016/j.talanta.2008.05.060. Epub 2008 Jun 17.
Herbicides are highly toxic for both human and animal health. The increased application of herbicides in agriculture during the last decades has resulted in the contamination of both soil and water. Herbicides, under illumination, can inhibit photosystem II electron transfer. Photosynthetic membranes isolated from higher plants and photosynthetic micro-organisms, immobilized and stabilized, can serve as a biorecognition element for a biosensor. The inhibition of photosystem II causes a reduced photoinduced production of hydrogen peroxide, which can be measured by a chemiluminescence reaction with luminol and the enzyme horseradish peroxidase. In the present work, a compact and portable sensing device that combines the production and detection of hydrogen peroxide in a single flow assay is proposed for herbicide detection.
除草剂对人类和动物健康都具有高毒性。在过去几十年中，农业中除草剂使用量的增加导致了土壤和水体的污染。除草剂在光照下会抑制光系统II的电子传递。从高等植物和光合微生物中分离出来的光合膜，经过固定和稳定化处理后，可作为生物传感器的生物识别元件。光系统II的抑制会导致光诱导产生的过氧化氢减少，而过氧化氢可以通过与鲁米诺和辣根过氧化物酶的化学发光反应来测量。在本研究中，提出了一种紧凑便携的传感装置，该装置在单一流动分析中结合了过氧化氢的产生和检测，用于除草剂检测。
