修饰核苷N(4),2'-O-二甲基胞苷（m(4)Cm）及其类似物的合成与溶液构象研究
Synthesis and solution conformation studies of the modified nucleoside N(4),2'-O-dimethylcytidine (m(4)Cm) and its analogues.
作者信息
Mahto Santosh K, Chow Christine S
机构信息
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
出版信息
Bioorg Med Chem. 2008 Oct 1;16(19):8795-800. doi: 10.1016/j.bmc.2008.09.016. Epub 2008 Sep 9.
Abstract
The dimethylated ribosomal nucleoside m(4)Cm and its monomethylated analogues Cm and m(4)C were synthesized. The conformations (syn vs anti) of the three modified nucleosides and cytidine were determined by CD and 1D NOE difference spectroscopy. The ribose sugar puckers were determined by the use of proton coupling constants. The position of modification (e.g., O vs N methylation) was found to have an effect on the sugar pucker of cytidine.
摘要
合成了二甲基化的核糖体核苷m(4)Cm及其单甲基化类似物Cm和m(4)C。通过圆二色光谱（CD）和一维核Overhauser效应（1D NOE）差异光谱确定了这三种修饰核苷和胞苷的构象（顺式与反式）。利用质子耦合常数确定了核糖的糖环构象。发现修饰位置（例如，O-甲基化与N-甲基化）对胞苷的糖环构象有影响。
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