Kotrikadze N, Alibegashvili M, Zibzibadze M, Abashidze N, Chigogidze T, Managadze L, Artsivadze K
Faculty of Exact and Natural Sciences, Institute of Biology, Iv. Javakhishvili Tbilisi State University, Tbilisi, Georgea.
Exp Oncol. 2008 Sep;30(3):244-7.
To investigate the antioxidant enzyme system in blood of men with benign hyperplasia of prostate (BHP) and with prostate adenocarcinoma (CaP).
The spectrophotometrical methods were applied to study content and activity changes of superoxide-dismutase (SOD), catalase (CAT), ceruloplasmin (Cp), tripeptide glutathione (GSH), glutathione-peroxidase (GSH-Px), and glutathione-reductase (GR). Lipid peroxidation was evaluated using thiobarbituric acid (TBA)-test. Blood plasma and erythrocytes of men with prostate tumors served as the material for the studies; n = 15 for each group.
SOD activity was increased in BHP (24.65 +/- 1.20 U/microl) and decreased in CaP (11.45 +/- 0.89 U/microl), CAT activity remained unaltered in BHP (12.41 +/- 0.85 mcat/ml) and was slightly declined in CaP (9.52 +/- 0.56 mcat/ml). Cp was increased in both kind of tumors, especially in CaP (54.27 +/- 7.22 mg%), as well as GSH (0.736 +/- 0.07 microM/l) and GR (0.031 +/- 0.002 microM/g.Hemogl/min). GSH-Px was sharply increased in BHP (0.67 +/- 0.05 microM/g.Hemogl/min) and reduced in CaP (0.16 +/- 0.01 microM/g.Hemogl/min).
The development of BHP reflects relatively weakly on blood system as activity and content of antioxidant enzymes are not revealing marked changes in this disease. The significant changes are revealed in case of CaP, showing the reduced functional state of blood antioxidant enzyme system.
研究前列腺良性增生（BHP）和前列腺腺癌（CaP）男性患者血液中的抗氧化酶系统。
采用分光光度法研究超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、铜蓝蛋白（Cp）、三肽谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽还原酶（GR）的含量及活性变化。用硫代巴比妥酸（TBA）试验评估脂质过氧化。前列腺肿瘤男性患者的血浆和红细胞作为研究材料；每组n = 15。
BHP患者SOD活性升高（24.65±1.20 U/μl），CaP患者SOD活性降低（11.45±0.89 U/μl）；BHP患者CAT活性保持不变（12.41±0.85 mcat/ml），CaP患者CAT活性略有下降（9.52±0.56 mcat/ml）。两种肿瘤患者的Cp均升高，尤其是CaP患者（54.27±7.22 mg%），GSH（0.736±0.07 μmol/l）和GR（0.031±0.002 μmol/g·Hemogl/min）也升高。BHP患者GSH-Px急剧升高（0.67±0.05 μmol/g·Hemogl/min），CaP患者GSH-Px降低（0.16±0.01 μmol/g·Hemogl/min）。
BHP的发展对血液系统影响相对较弱，因为抗氧化酶的活性和含量在该疾病中未显示出明显变化。CaP患者则出现显著变化，表明血液抗氧化酶系统功能状态降低。
Zotero 插件