Webb Natasha, Connolly Geoff, Tellam Judy, Yap Alpha S, Khanna Rajiv
Australian Centre for Vaccine Development and Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Australia.
PLoS One. 2008 Sep 22;3(9):e3254. doi: 10.1371/journal.pone.0003254.
Previous studies have indicated that Epstein-Barr virus (EBV) can modulate the Wnt pathway in virus-infected cells and this effect is mediated by EBV-encoded oncogene latent membrane protein 1 (LMP1). Here we have reassessed the role of LMP1 in regulating the expression of various mediators of the canonical Wnt cascade. Contradicting the previous finding, we found that the levels of E-cadherin, beta-catenin, Glycogen Synthase Kinase 3ss (GSK3beta), axin and alpha-catenin were not affected by the expression of LMP1 sequences from normal B cells or nasopharyngeal carcinoma. Moreover, we also show that LMP1 expression had no detectable effect on the E-cadherin and beta-catenin interaction and did not induce transcriptional activation of beta-catenin. Taken together these studies demonstrate that EBV-mediated activation of Wnt pathway is not dependent on the expression of LMP1.
先前的研究表明，爱泼斯坦-巴尔病毒（EBV）可调节病毒感染细胞中的Wnt信号通路，且这种效应由EBV编码的癌基因潜伏膜蛋白1（LMP1）介导。在此，我们重新评估了LMP1在调节经典Wnt级联反应各种介质表达中的作用。与先前的发现相反，我们发现E-钙黏蛋白、β-连环蛋白、糖原合酶激酶3β（GSK3β）、轴抑制蛋白和α-连环蛋白的水平不受正常B细胞或鼻咽癌来源的LMP1序列表达的影响。此外，我们还表明，LMP1的表达对E-钙黏蛋白和β-连环蛋白的相互作用没有可检测到的影响，也不会诱导β-连环蛋白的转录激活。综上所述，这些研究表明EBV介导的Wnt信号通路激活不依赖于LMP1的表达。
