Choi Eun Jeong, Kim Taehee
Plant Resources Research Institute, Duksung Women's University, Seoul, 132-714, Korea.
Arch Pharm Res. 2008 Sep;31(9):1115-9. doi: 10.1007/s12272-001-1277-3. Epub 2008 Sep 20.
We investigated the hepatic effects of daidzein on the 7,12-dimethylbenz(a)anthracene (DMBA)-induced enzymatic activity and expression of CYP1A1 and CYP1B1 in mice. Daidzein was administered orally to mice at 5 or 25 mg/kg BW for three weeks, after which DMBA (34 mg/kg BW) was administered intragastrically twice a week for two weeks. Twenty-four hours after the last DMBA treatment, the mice were sacrificed. DMBA induced CYP1 activity as well as the expression of CYP1A1 and CYP1B1. The catalytic activity of CYP1 enzymes was determined as 7-ethoxyresorufin-O-deethylase (EROD) activity. Each of these effects was significantly reduced by daidzein; however, the reduction was more pronounced for CYP1A1. Daidzein also exerted a negative effect on the transcription of CYP1A1 by AhR, similar to its effect on CYP1A1. The AhR-dependent inhibition of CYP1 by daidzein may explain the anticancer effects of daidzein.
我们研究了大豆苷元对7,12-二甲基苯并(a)蒽（DMBA）诱导的小鼠CYP1A1和CYP1B1酶活性及表达的肝脏影响。以5或25 mg/kg体重的剂量给小鼠口服大豆苷元，持续三周，之后每周两次以34 mg/kg体重的剂量给小鼠灌胃DMBA，持续两周。在最后一次DMBA处理24小时后，处死小鼠。DMBA诱导了CYP1活性以及CYP1A1和CYP1B1的表达。CYP1酶的催化活性通过7-乙氧基异吩恶唑酮-O-脱乙基酶（EROD）活性来测定。大豆苷元可显著降低上述每种效应；然而，对CYP1A1的降低更为明显。大豆苷元对AhR介导的CYP1A1转录也有负面影响，与其对CYP1A1的影响类似。大豆苷元对CYP1的AhR依赖性抑制作用可能解释了大豆苷元的抗癌作用。
