Plant Resources Research Institute, Duksung Women's University, Seoul 132-714, Republic of Korea.
Oncol Rep. 2012 Jul;28(1):291-6. doi: 10.3892/or.2012.1753. Epub 2012 Apr 2.
We investigated the effects of quercetin on 7,12-dimethylbenz(a)anthracene (DMBA)-induced oxidative stress and the expression of CYP1A1 and CYP1B1 in mice. Quercetin was administered orally to mice at 100 or 250 mg/kg BW for 18 days, after which DMBA (34 mg/kg BW) was administered intragastrically twice. Quercetin showed side effects such as increased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in DMBA-untreated mice. Also, quercetin induced AST and ALT in DMBA-treated, although this was not significantly different from levels in DMBA-treated controls. The thiobarbituric acid reactive substances (TBARS) value showed a tendency to decrease following quercetin treatment; these decreases were significantly greater in the DMBA-treated compared to the untreated groups. Also, catalase and superoxide dismutase (SOD) activities as well as their mRNA expression were increased by quercetin; this increase was more pronounced in DMBA-treated compared to untreated mice. DMBA induced CYP1 activity as well as expression of CYP1A1 and CYP1B1. Each of these effects was significantly reduced by quercetin; however, this reduction was observed for CYP1A1 at only the higher dose and for CYP1B1 at both doses. These data suggest that quercetin shows antioxidant activity against DMBA-induced oxidative stress. Moreover, its regulation of CYP1A1 and CYP1B1 suggests the potential of quercetin as an anticancer supplement.
我们研究了槲皮素对 7,12-二甲基苯并(a)蒽（DMBA）诱导的氧化应激以及 CYP1A1 和 CYP1B1 在小鼠中的表达的影响。槲皮素以 100 或 250mg/kg BW 的剂量经口给予小鼠 18 天，之后两次经胃内给予 DMBA（34mg/kg BW）。在未用 DMBA 处理的小鼠中，槲皮素表现出副作用，如天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）升高。此外，槲皮素在 DMBA 处理的小鼠中诱导了 AST 和 ALT，尽管与 DMBA 处理的对照组相比，这并没有显著差异。硫代巴比妥酸反应物质（TBARS）值在槲皮素处理后呈现下降趋势；与未处理组相比，DMBA 处理组的下降更为显著。此外，CAT 和超氧化物歧化酶（SOD）的活性及其 mRNA 表达也被槲皮素增加；与未处理的小鼠相比，DMBA 处理的小鼠增加更为明显。DMBA 诱导 CYP1 活性以及 CYP1A1 和 CYP1B1 的表达。槲皮素显著降低了每种这些效应；然而，这种降低仅在较高剂量时观察到 CYP1A1，而在两种剂量下均观察到 CYP1B1。这些数据表明，槲皮素对 DMBA 诱导的氧化应激表现出抗氧化活性。此外，其对 CYP1A1 和 CYP1B1 的调节表明槲皮素作为一种抗癌补充剂的潜力。
