Yu Haifeng, Jia Shiru
Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin 300457, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Jun;24(6):1029-34.
The extracellular polysaccharides (EPS) of N. flagelliforme were purified by DEAE anion exchange chromatography and Sephadex G100 gel filtration chromatography. And two main components named NFPS1 and NFPS2 were obtained respectively. The physico-chemical characteristics of NFPS2 were analyzed and compared with NFPS0, which was obtained from field colony of N. flagelliforme. These results showed that both of NFPS2 and NFPSO were composed of four monosaccharides: glucose, xylose, galactose and mannose. The apparent molecular weight of NFPS2 and NFPS0 was estimated to be 2.79 x 10(5), 2.26 x 10(5) respectively. They are non-sulfated polysaccharides, free of protein and nuclear acid. The thermal analysis indicated that there was a decomposition peak at 245 degrees C in thermogravimetric (TG) curves. However, the microstructure analysis showed that they had different porous structures.
通过DEAE阴离子交换色谱和Sephadex G100凝胶过滤色谱法对发菜的胞外多糖（EPS）进行了纯化。分别获得了两个主要成分，命名为NFPS1和NFPS2。分析了NFPS2的理化特性，并与从发菜田间菌落中获得的NFPS0进行了比较。这些结果表明，NFPS2和NFPS0均由四种单糖组成：葡萄糖、木糖、半乳糖和甘露糖。NFPS2和NFPS0的表观分子量估计分别为2.79×10⁵、2.26×10⁵。它们是无硫酸化的多糖，不含蛋白质和核酸。热分析表明，热重（TG）曲线在245℃时有一个分解峰。然而，微观结构分析表明它们具有不同的多孔结构。
