Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, PR China.
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Carbohydr Polym. 2014 May 25;105:145-51. doi: 10.1016/j.carbpol.2014.01.061. Epub 2014 Jan 29.
The influences of different wavelengths of light (red 660nm, yellow 590nm, green 520nm, blue 460nm, purple 400nm) and white light on extracellular polysaccharide (EPS) and capsular polysaccharide (CPS) production by Nostoc flagelliforme in liquid culture were demonstrated in this study. The results showed that, compared with white light, red and blue lights significantly increased both EPS and CPS production while yellow light reduced their production; purple and green lights stimulated EPS production but inhibited CPS formation. Nine constituent monosaccharides and one uronic acid were detected in both EPS and CPS, and their ratios showed significant differences among treatment with different light wavelengths. However, the advanced structure of EPS and CPS from various light conditions did not present obvious difference through Fourier transform infrared spectroscopy and X-ray diffraction characterization. These findings establish a basis for development of high-yielding polysaccharide production process and understanding their regulation.
本研究旨在探讨不同波长的光（红 660nm、黄 590nm、绿 520nm、蓝 460nm、紫 400nm）和白光对液体培养中的发菜胞外多糖（EPS）和荚膜多糖（CPS）生产的影响。结果表明，与白光相比，红光和蓝光显著增加了 EPS 和 CPS 的产量，而黄光则降低了它们的产量；紫光和绿光刺激 EPS 产量，但抑制 CPS 形成。在 EPS 和 CPS 中均检测到 9 种组成单糖和 1 种糖醛酸，其比例在不同波长的光处理之间存在显著差异。然而，通过傅里叶变换红外光谱和 X 射线衍射表征，不同光照条件下 EPS 和 CPS 的高级结构并未呈现明显差异。这些发现为开发高产多糖生产工艺和理解其调控机制奠定了基础。
