Yilmaz Atilla, Schaller Tina, Cicha Iwona, Altendorf Regina, Stumpf Christian, Klinghammer Lutz, Ludwig Josef, Daniel Werner G, Garlichs Christoph D
Medical Clinic II, University Hospital of Erlangen, Erlangen, Germany.
Clin Sci (Lond). 2009 Feb;116(4):353-63. doi: 10.1042/CS20080392.
DCs (dendritic cells) are present in atherosclerotic lesions leading to vascular inflammation, and the number of vascular DCs increases during atherosclerosis. Previously, we have shown that the levels of circulating DCPs (DC precursors) are reduced in acute coronary syndromes through vascular recruitment. In the present study, we have investigated whether DCP levels are also reduced in stable CAD (coronary artery disease). The levels of circulating mDCPs (myeloid DCPs), pDCPs (plasmacytoid DCPs) and tDCP (total DCPs) were investigated using flow cytometry in 290 patients with suspected stable CAD. A coronary angiogram was used to evaluate a CAD score for each patient as follows: (i) CAD excluded (n=57); (ii) early CAD (n=63); (iii) moderate CAD (n=85); and (iv) advanced CAD (n=85). Compared with controls, patients with advanced stable CAD had lower HDL (high-density lipoprotein)-cholesterol (P=0.03) and higher creatinine (P=0.003). In advanced CAD, a significant decrease in circulating mDCPs, pDCPs and tDCPs was observed (each P<0.001). A significant inverse correlation was observed between the CAD score and mDCPs, pDCPs or tDCPs (each P<0.001). Patients who required percutaneous coronary intervention or coronary artery bypass grafting had less circulating mDCPs, pDCPs and tDCPs than controls (each P<0.001). Multiple stepwise logistic regression analysis suggested mDCPs, pDCPs and tDCPs as independent predictors of CAD. In conclusion, we have shown that patients with stable CAD have significantly lower levels of circulating DCPs than healthy individuals. Their decrease appears to be an independent predictor of the presence of, and subsequent therapeutic procedure in, stable CAD.
树突状细胞（DCs）存在于导致血管炎症的动脉粥样硬化病变中，并且在动脉粥样硬化过程中血管DC的数量会增加。此前，我们已经表明，急性冠脉综合征中循环DC前体（DCPs）的水平通过血管募集而降低。在本研究中，我们调查了稳定型冠心病（CAD）患者的DCP水平是否也降低。我们使用流式细胞术对290例疑似稳定型CAD患者的循环髓样DCP（mDCPs）、浆细胞样DCP（pDCPs）和总DCP（tDCP）水平进行了检测。采用冠状动脉造影对每位患者的CAD评分进行评估，具体如下：（i）排除CAD（n = 57）；（ii）早期CAD（n = 63）；（iii）中度CAD（n = 85）；（iv）重度CAD（n = 85）。与对照组相比，重度稳定型CAD患者的高密度脂蛋白（HDL）胆固醇水平较低（P = 0.03），肌酐水平较高（P = 0.003）。在重度CAD患者中，观察到循环mDCPs、pDCPs和tDCPs显著降低（均P < 0.001）。CAD评分与mDCPs、pDCPs或tDCPs之间存在显著的负相关（均P < 0.001）。需要经皮冠状动脉介入治疗或冠状动脉旁路移植术的患者，其循环mDCPs、pDCPs和tDCPs水平低于对照组（均P < 0.001）。多因素逐步逻辑回归分析表明，mDCPs、pDCPs和tDCPs是CAD的独立预测因子。总之，我们已经表明稳定型CAD患者的循环DCPs水平显著低于健康个体。其降低似乎是稳定型CAD存在及后续治疗程序的独立预测因子。
