Zhuang Jianhui, Han Yang, Xu Dachun, Zhu Guofu, Singh Shekhar, Chen Luoman, Zhu Mengyun, Chen Wei, Xu Yawei, Li Xiankai
Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Department of Pathology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
BMC Cardiovasc Disord. 2017 Oct 18;17(1):270. doi: 10.1186/s12872-017-0702-3.
While specific patterns of circulating dendritic cells (DCs) and monocytes are associated with the incidence of coronary artery disease, the characterization of circulating DC and monocyte subsets in patients with different stages of atherosclerosis remains unclear.
Forty-eight patients with unstable angina pectoris (UAP) diagnosed by angiography were enrolled. Likewise, 31 patients with ST-segment elevation myocardial infarction (STEMI) were enrolled and confirmed with the presence of thrombosis by angiography. Plaque features of 48 UAP patients were evaluated at the culprit lesions by OCT. Circulating myeloid DCs (mDCs), plasmacytoid DCs (pDCs) and monocyte subsets were analyzed using flow cytometry.
The proportions and absolute counts of mDC2s, which specifically express CD141 and possess the ability to activate CD8+ T lymphocytes, significantly decreased in patients with UAP and STEMI when compared with controls (0.08 × 10 ± 0.05 × 104/ml and 0.08 × 10 ± 0.06 × 104/ml vs. 0.11 × 10 ± 0.06 × 104/ml, p = 0.027). On the other hand, patients with UAP and STEMI had significantly higher proportions and counts of Mon2 subsets. In the OCT subgroup, patients with thin-cap fibroatheroma (TCFA) had higher proportions and absolute number of Mon2 (11.96% ± 4.27% vs. 9.42% ± 4.05%, p = 0.034; 5.17 × 104/ml ± 1.92 × 104/ml vs. 3.53 × 104/ml ± 2.65 × 104/ml, p = 0.045) than those without TCFA. However, there was no remarkable difference in mDC2s between patients with and without TCFA.
Circulating Mon2 appears to be a promising marker for the severity of atherosclerotic plaque.
虽然循环树突状细胞（DCs）和单核细胞的特定模式与冠状动脉疾病的发病率相关，但不同动脉粥样硬化阶段患者循环DC和单核细胞亚群的特征仍不清楚。
纳入48例经血管造影诊断为不稳定型心绞痛（UAP）的患者。同样，纳入31例ST段抬高型心肌梗死（STEMI）患者，并经血管造影证实存在血栓形成。通过光学相干断层扫描（OCT）评估48例UAP患者罪犯病变处的斑块特征。使用流式细胞术分析循环髓样DC（mDCs）、浆细胞样DC（pDCs）和单核细胞亚群。
与对照组相比，UAP和STEMI患者中特异性表达CD141并具有激活CD8 + T淋巴细胞能力的mDC2s的比例和绝对计数显著降低（0.08×10±0.05×104/ml和0.08×10±0.06×104/ml vs. 0.11×10±0.06×104/ml，p = 0.027）。另一方面，UAP和STEMI患者的Mon2亚群比例和计数显著更高。在OCT亚组中，薄帽纤维粥样斑块（TCFA）患者的Mon2比例和绝对数量高于无TCFA患者（11.96%±4.27% vs. 9.42%±4.05%，p = 0.034；5.17×104/ml±1.92×104/ml vs. 3.53×104/ml±2.65×104/ml，p = 0.045）。然而，有TCFA和无TCFA患者的mDC2s之间没有显著差异。
循环Mon2似乎是动脉粥样硬化斑块严重程度的一个有前景的标志物。
