Pedersen Mikael E, Szewczyk Bernadeta, Stachowicz Katarzyna, Wieronska Joanna, Andersen Jacob, Stafford Gary I, van Staden Johannes, Pilc Andrzej, Jäger Anna K
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, 2 Universitetsparken, 2100 Copenhagen O, Denmark.
J Ethnopharmacol. 2008 Oct 28;119(3):542-8. doi: 10.1016/j.jep.2008.08.030. Epub 2008 Sep 10.
The four South African medicinal plants Agapanthus campanulatus (AC), Boophone distica (BD), Mondia whitei (MW) and Xysmalobium undulatum (XU) are used in traditional medicine to treat depression.
To evaluate the effect of ethanolic extracts of the plants in models for depression.
The extracts were screened for affinity for the serotonin transporter (SERT) in the [(3)H]-citalopram-binding assay. The inhibitory potency of the extracts towards the SERT, the noradrenalin transporter (NAT) and the dopamine transporter (DAT) were determined in a functional uptake inhibition assay. Antidepressant-like effects of the extracts were investigated using the tail suspension test (TST) and the forced swim test in both rats (rFST) and mice (mFST).
All four plants showed affinity for SERT in the binding assay. AC and BD showed functional inhibition of SERT, NAT and DAT, MW affected SERT while XU showed no effect. BD showed significant effect in the TST and in the mFST/rFST, AC showed significant effect in mFST, MW showed significant effect in the rFST and XU showed significant effect in the mFST.
In this study we have demonstrated the antidepressant activity of four South African medicinal plants in vitro and in vivo, supporting their rational use in traditional medicine.
南非的四种药用植物蓝花君子兰（AC）、非洲天门冬（BD）、白叶蒙地亚（MW）和波叶乳突果（XU）在传统医学中用于治疗抑郁症。
评估这些植物乙醇提取物在抑郁症模型中的作用。
采用[³H] - 西酞普兰结合试验筛选提取物对5-羟色胺转运体（SERT）的亲和力。在功能性摄取抑制试验中测定提取物对SERT、去甲肾上腺素转运体（NAT）和多巴胺转运体（DAT）的抑制效力。使用大鼠悬尾试验（TST）和强迫游泳试验（大鼠强迫游泳试验[rFST]和小鼠强迫游泳试验[mFST]）研究提取物的抗抑郁样作用。
在结合试验中，所有四种植物均显示出对SERT的亲和力。AC和BD对SERT、NAT和DAT有功能性抑制作用，MW影响SERT，而XU无作用。BD在TST以及mFST/rFST中显示出显著作用，AC在mFST中显示出显著作用，MW在rFST中显示出显著作用，XU在mFST中显示出显著作用。
在本研究中，我们已证明四种南非药用植物在体外和体内均具有抗抑郁活性，支持它们在传统医学中的合理应用。
