小鼠单根神经支配和去神经支配的骨骼肌细胞中乙酰胆碱诱导挛缩的细胞外钙离子依赖性

The external Ca(2+)-dependence of acetylcholine-induced contracture in single innervated and denervated skeletal muscle cells in mice.

作者信息

Nojima H, Kimura I, Kimura M

机构信息

Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.

出版信息

Jpn J Pharmacol. 1991 May;56(1):109-12. doi: 10.1254/jjp.56.109.

DOI:10.1254/jjp.56.109

PMID:1880980

Abstract

We examined morphological differences and compared the Ca(2+)-dependence between acetylcholine (ACh)-contractures in normal and denervated skeletal muscle cells. ACh (1 microM - 1 mM) contracted the normal cells into a jackknife-shape. The higher the concentration of external Ca2+, the greater the ratio of responding cells/total cells observed and the sharper the angles of the "jackknives". ACh contracted the denervated cells into a compression-form, and the contraction was dependent on the external Ca2+. These results indicate that ACh-contractures in both normal and denervated cells are external Ca(2+)-dependent.

摘要

我们研究了正常和去神经支配的骨骼肌细胞中乙酰胆碱（ACh）诱导的挛缩之间的形态学差异，并比较了其对钙离子（Ca²⁺）的依赖性。乙酰胆碱（1微摩尔 - 1毫摩尔）可使正常细胞收缩成折叠刀状。细胞外钙离子浓度越高，观察到的反应细胞与总细胞的比例越大，“折叠刀”的角度越尖锐。乙酰胆碱使去神经支配的细胞收缩成压缩状，且这种收缩依赖于细胞外钙离子。这些结果表明，正常和去神经支配细胞中的乙酰胆碱诱导挛缩均依赖于细胞外钙离子。