Masuda Y, Yamamori Y
Division of Toxicology, Niigata College of Pharmacy, Japan.
Jpn J Pharmacol. 1991 Jun;56(2):133-42. doi: 10.1254/jjp.56.133.
Lipid peroxidation was assessed histologically and biochemically in hemoglobin-free perfused rat livers using two different types of stimulators. The Schiff reaction of fuchsin with cellular aldehydes was used as a histological index for lipid peroxidation. t-Butyl hydroperoxide (BHP, 0.8 mM) infusion caused a rapid and sustained release of thiobarbituric acid reactive substances (TBARS) into the effluent perfusate for up to 60 min, which was accompanied by lactate dehydrogenase (LDH) leakage after 30 min. The Schiff positive foci were initially restricted to periportal zones and spread with time to whole areas, accompanied by periportal necrosis. Coinfusion of diphenyl-p-phenylenediamine suppressed the TBARS release, with negative fuchsin staining, but the LDH leakage was unaffected. Under retrograde perfusion, BHP produced pericentral staining and necrosis. With 2.5 mM ADP-100 microM Fe3+, little TBARS was released up to 60 min, even though the hepatic TBARS levels increased considerably by this time. By 90 min, marked TBARS release occurred, but LDH leakage remained low. Irrespective of the direction of perfusion, pericentral hepatocytes became Schiff positive after 30 min. The fuchsin staining method may be useful for detecting peroxidized zones of the liver lobules.
使用两种不同类型的刺激剂，通过组织学和生物化学方法对无血红蛋白灌注的大鼠肝脏中的脂质过氧化进行评估。品红与细胞醛的席夫反应被用作脂质过氧化的组织学指标。输注叔丁基过氧化氢（BHP，0.8 mM）会导致硫代巴比妥酸反应性物质（TBARS）迅速且持续地释放到流出的灌注液中，持续长达60分钟，30分钟后伴有乳酸脱氢酶（LDH）泄漏。席夫阳性灶最初局限于门周区域，随后随时间扩展至整个区域，并伴有门周坏死。同时输注二苯基对苯二胺可抑制TBARS释放，品红染色呈阴性，但LDH泄漏不受影响。在逆行灌注下，BHP产生中央周围染色和坏死。使用2.5 mM ADP - 100 microM Fe3+时，在60分钟内几乎没有TBARS释放，尽管此时肝脏TBARS水平已显著升高。到90分钟时，出现明显的TBARS释放，但LDH泄漏仍然很低。无论灌注方向如何，30分钟后中央周围肝细胞品红染色呈阳性。品红染色法可能有助于检测肝小叶的过氧化区域。
