Kocogullari C U, Emmiler M, Cemek M, Sahin O, Aslan A, Ayva E, Tur L, Buyukokuroglu M E, Demirkan I, Cekirdekci A
Department of Cardiovascular Surgery, Afyon Kocatepe University Medical Faculty, Afyon, Turkey.
Thorac Cardiovasc Surg. 2008 Oct;56(7):406-11. doi: 10.1055/s-2008-1038731. Epub 2008 Sep 22.
The aim of this experimental study was to investigate the possible protective effect of dantrolene on neuronal injury induced by aortic ischemia/reperfusion (I/R).
Nineteen rabbits were divided into three groups: sham (group 1, n = 5, no I/R), control (group 2, n = 7, only I/R) and dantrolene (group 3, n = 7, dantrolene + I/R). Abdominal aortic occlusion between the renal arteries and iliac bifurcations was carried out for 30 min. The spinal cord functions of the subjects were assessed using the Tarlov Scale. Blood and cord tissue samples were taken for biochemical and histopathological evaluation.
Tarlov scores in group 3 were significantly higher than in group 2 ( P < 0.05). In group 3, the MDA levels of the spinal cord decreased significantly compared to those of group 2 ( P < 0.05). In rabbits with I/R (group 2), the GSH levels of the spinal cord decreased significantly compared to those of group 1 ( P < 0.01), but dantrolene pretreatment significantly prevented a decrease in GSH levels. Histopathological examination showed that group 3 had less vascular proliferation, hemorrhage, edema and neuron loss than group 2.
It was concluded that dantrolene applied after ischemia might help protect the spinal cord against ischemia/reperfusion injury.
本实验研究旨在探讨丹曲林对主动脉缺血/再灌注（I/R）诱导的神经元损伤的可能保护作用。
将19只家兔分为三组：假手术组（第1组，n = 5，无I/R）、对照组（第2组，n = 7，仅I/R）和丹曲林组（第3组，n = 7，丹曲林 + I/R）。在肾动脉和髂动脉分叉之间进行腹主动脉阻断30分钟。使用塔尔洛夫量表评估受试者的脊髓功能。采集血液和脊髓组织样本进行生化和组织病理学评估。
第3组的塔尔洛夫评分显著高于第2组（P < 0.05）。与第2组相比，第3组脊髓的丙二醛水平显著降低（P < 0.05）。在I/R家兔（第2组）中，脊髓的谷胱甘肽水平与第1组相比显著降低（P < 0.01），但丹曲林预处理显著防止了谷胱甘肽水平的降低。组织病理学检查显示，第3组的血管增殖、出血、水肿和神经元丢失比第2组少。
得出的结论是，缺血后应用丹曲林可能有助于保护脊髓免受缺血/再灌注损伤。
