Cengiz Ustuner M, Degirmenci Irfan, Kiran Ismail, Ozden Hilmi, Civi Kismet, Ustuner Derya, Gunes Hasan V
Department of Medical Biology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey.
Saudi Med J. 2008 Sep;29(9):1241-6.
To determine the protective effects of a fungal metabolite of demethoxyviridine (DMV) and its derivative, 1-alpha-hydroxy-DMV in the livers of 2-month-old male Spraque-Dawley rats treated with diethylnitrosamine (DEN) and 2-acetylaminflourene (2-AAF).
This study was performed in the Department of Medical Biology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey from May 2006. Animals were divided into 10 groups. Those were the control, olive oil, dimethyl sulfoxide (DMSO), DMV, 1-alpha-hydroxy-DMV, DEN, 2-AAF, DEN+2-AAF, DEN+2-AAF+DMV, and DEN+2-AAF+1-alpha-hydroxy-DMV-treated animal groups. The liver microsomes were prepared from rats and the levels of expression of cytochrome P450 1A2 (CYP1A2) enzymes were determined with western blot technique. The liver tissue slides were evaluated histopathologically with hematoxylin and eosin staining and immunohistochemically for Harvey-retrovirus associated DNA sequences (Ha-Ras), glutathione S- transferase (GST-p), and connexion-32 (Cx32) proteins.
Notably, there were no appreciable differences in CYP1A2 level among control, olive oil, and DMSO-treated animals. The CYP1A2 level was significantly decreased in 2-AAF, DEN+2-AAF, DEN, DEN+2-AAF+DMV, DEN+2-AAF+1-alpha-hydroxy-DMV, 1-alpha-hydroxy-DMV, and DMV-treated animals as compared to the control. Most prenoplastic focus was found in DEN+2-AAF treated group.
Demethoxyviridine and 1-alpha-hidroksi-DMV had protective effect in the livers of DEN, 2-AAF and DEN+2-AAF induced rats.
确定去甲氧基维立定（DMV）的一种真菌代谢产物及其衍生物1-α-羟基-DMV对用二乙基亚硝胺（DEN）和2-乙酰氨基芴（2-AAF）处理的2月龄雄性斯普拉格-道利大鼠肝脏的保护作用。
本研究于2006年5月在土耳其埃斯基谢希尔奥斯曼加齐大学医学院医学生物学系进行。动物分为10组。分别为对照组、橄榄油组、二甲基亚砜（DMSO）组、DMV组、1-α-羟基-DMV组、DEN组、2-AAF组、DEN + 2-AAF组、DEN + 2-AAF + DMV组和DEN + 2-AAF + 1-α-羟基-DMV处理的动物组。从大鼠制备肝脏微粒体，并用蛋白质印迹技术测定细胞色素P450 1A2（CYP1A2）酶的表达水平。肝脏组织切片用苏木精和伊红染色进行组织病理学评估，并用免疫组织化学方法检测哈维逆转录病毒相关DNA序列（Ha-Ras）、谷胱甘肽S-转移酶（GST-p）和连接蛋白-32（Cx32）蛋白。
值得注意的是，对照组、橄榄油组和DMSO处理组动物的CYP1A2水平没有明显差异。与对照组相比，2-AAF组、DEN + 2-AAF组、DEN组、DEN + 2-AAF + DMV组、DEN + 2-AAF + 1-α-羟基-DMV组、1-α-羟基-DMV组和DMV处理组动物的CYP1A2水平显著降低。在DEN + 2-AAF处理组中发现的癌前病灶最多。
去甲氧基维立定和1-α-羟基-DMV对DEN、2-AAF和DEN + 2-AAF诱导的大鼠肝脏具有保护作用。
