Rahmat A, Ngah W Z, Shamaan N A, Gapor A, Abdul Kadir K
Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur.
Nutrition. 1993 May-Jun;9(3):229-32.
The effects of long-term administration of tocotrienol on hepatocarcinogenesis in rats induced by diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) were investigated by determining the activities of gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), glutathione S-transferases (GSTs), and glutathione (GSH) levels in blood and liver. Twenty-eight male 7- to 8-wk-old Rattus norwegicus rats, weighing 120-160 g, were used in this study. The rats were divided into four treatment groups: a control group on a basal diet, a group fed a basal diet supplemented with tocotrienol (30 mg/kg food), a group treated with DEN/AAF, and a group treated with DEN/AAF and fed a diet supplemented with tocotrienol (30 mg/kg food). Blood was collected monthly, and GGT, ALP, and GSH levels were determined. The rats were killed after 9 mo, and the livers were examined morphologically. Grayish white nodules (2/liver) were found in all the DEN/AAF-treated rats (n = 10), but only one of the rats treated with DEN/AAF and supplemented with tocotrienol (n = 6) had liver nodules. A significant increase in the level of blood and liver GSH, ALP, and GGT activities was observed in the DEN/AAF-treated rats. Liver GSTs were similarly increased with DEN/AAF treatment. Tocotrienol supplementation attenuated the impact of the carcinogens in the rats.
通过测定血液和肝脏中γ-谷氨酰转肽酶（GGT）、碱性磷酸酶（ALP）、谷胱甘肽S-转移酶（GSTs）的活性以及谷胱甘肽（GSH）水平，研究了长期给予生育三烯酚对二乙基亚硝胺（DEN）和2-乙酰氨基芴（AAF）诱导的大鼠肝癌发生的影响。本研究使用了28只7至8周龄的雄性挪威大鼠，体重120 - 160克。大鼠被分为四个治疗组：基础饮食对照组、喂食补充生育三烯酚（30毫克/千克食物）的基础饮食组、接受DEN/AAF处理的组以及接受DEN/AAF处理并喂食补充生育三烯酚（30毫克/千克食物）的组。每月采集血液，测定GGT、ALP和GSH水平。9个月后处死大鼠，对肝脏进行形态学检查。在所有接受DEN/AAF处理的大鼠（n = 10）中均发现灰白色结节（每个肝脏2个），但在接受DEN/AAF处理并补充生育三烯酚的大鼠中（n = 6）只有一只出现肝脏结节。在接受DEN/AAF处理的大鼠中，血液和肝脏中的GSH水平、ALP和GGT活性显著增加。DEN/AAF处理同样使肝脏GSTs增加。补充生育三烯酚减轻了致癌物对大鼠的影响。
