Swarna S R, Parija Subhash Chandra
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical education and Research, Pondicherry, India.
Rev Inst Med Trop Sao Paulo. 2008 Jul-Aug;50(4):233-6. doi: 10.1590/s0036-46652008000400009.
The aim of the present study is to evaluate cyst wall and protoscolex as an alternate source of antigen in serodiagnosis of cystic echinococcosis (CE). A total of 90 blood samples, 30 each of confirmed CE cases, disease controls and healthy controls were collected. Dot-ELISA using cyst wall, protoscolex and cyst fluid were used to demonstrate anti-hydatid antibodies. The sensitivity of Dot-ELISA using cyst wall, protoscolex and cyst fluid was 96.66%, 86.66% and 93.33% respectively and the specificity of the assay was 70% for Dot-ELISA using cyst fluid, protoscolex and cyst wall antigens. Results of the present study show that cyst wall and protoscolex can also be an useful source of antigen in detection of hydatid antibodies in the serodiagnosis of CE.
本研究的目的是评估囊壁和原头节作为囊性棘球蚴病（CE）血清诊断中抗原的替代来源。共采集了90份血样，其中确诊的CE病例、疾病对照和健康对照各30份。使用囊壁、原头节和囊液进行斑点酶联免疫吸附测定（Dot-ELISA）以检测抗包虫抗体。使用囊壁、原头节和囊液进行Dot-ELISA的敏感性分别为96.66%、86.66%和93.33%，使用囊液、原头节和囊壁抗原进行Dot-ELISA检测的特异性为70%。本研究结果表明，在CE血清诊断中检测包虫抗体时，囊壁和原头节也可作为有用的抗原来源。
