Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Asian Pac J Trop Med. 2013 Sep;6(9):723-7. doi: 10.1016/S1995-7645(13)60126-1.
To explore the serodiagnosis of hydatid cyst in human using different antigens of sheep (hydatid fluid, Somatic and Excretory/secretory antigens of protoscolex) by ELISA and compares this result with commercial human ELISA kit.
One hundred blood samples from patients with history of severe abdominal pain and eosinophilia were obtained. Ten serum samples were obtained from surgically and pathologically confirmed cystic echinococcosis patients from Mashhad university hospital as positive control and 5 serum samples from infant under one year old as negative control. Blood samples were centrifuged at 3 000μg at 20 °C for 15 min and sera were stored at -20 °C. First, these samples were tested for the presence of antibody by commercial human ELISA. Then, ELISA was developed on microplates coated with hydatid fluid, Somatic and Excretory/secretory antigens of protoscolex of sheep.
The results of this study as analyzed by Kappa test showed that, hydatid fluid antigen could be used as a precise source of detection in indirect ELISA test.
Hydatid fluid in comparison with Excretory-secretory and somatic antigens showed more compatibility agreement in kappa test which can be used for further studies in development of any ELISA test for diagnosis of human hydatidosis.
通过酶联免疫吸附试验（ELISA）用绵羊（包虫液、体抗原和排泄/分泌原头蚴抗原）的不同抗原探索人体包虫囊的血清学诊断，并将结果与市售的人类 ELISA 试剂盒进行比较。
从马什哈德大学医院接受过严重腹痛和嗜酸性粒细胞增多病史的 100 名患者中获得 100 份血样。10 份血清样本取自经手术和病理证实的囊型包虫病患者，作为阳性对照，5 份血清样本取自 1 岁以下的婴儿，作为阴性对照。将血液在 20°C 下以 3000μg 的速度离心 15 分钟，将血清储存在-20°C。首先，通过市售的人类 ELISA 检测这些样本中抗体的存在。然后，在包虫液、体抗原和排泄/分泌原头蚴抗原包被的微孔板上进行 ELISA。
通过 Kappa 检验对本研究的结果进行分析表明，包虫液抗原可作为间接 ELISA 检测的精确检测源。
与排泄/分泌和体抗原相比，包虫液在 Kappa 检验中显示出更高的一致性，可用于进一步研究开发用于诊断人类包虫病的任何 ELISA 检测方法。
