Gu Lianzhi, Pandey Vikas, Geenen David L, Chowdhury Shamim A K, Piano Mariann R
Department of Medical-Surgical Nursing, University of Illinois at Chicago, Chicago, United States.
Eur J Heart Fail. 2008 Nov;10(11):1057-64. doi: 10.1016/j.ejheart.2008.08.009. Epub 2008 Sep 24.
To determine the effects of cigarette smoke (CS) exposure on the expression/activation of mitogen-activated protein kinases (MAPKs) (extracellular signal-regulated kinase [ERK1/2], p38-kinase [p38] and c-Jun NH2-terminal protein kinase [JNK]), norepinephrine (NE) levels and myocardial structure and function.
Rats were randomised to two groups: CS-exposed (n=12) or room air (CON) (n=10). After 5 weeks, the animals underwent echocardiography with pulse-wave Doppler flow measurements. Hearts were removed for microscopy and Western blot analysis.
CS exposure was associated with significant increases in NE urinary levels and larger ventricular dimensions (mm) (CON=left ventricular end diastolic dimension [LVEDD] 7.99+/-0.10, LV end systolic dimension [LVESD] 4.55+/-0.20, CS=LVEDD 8.3+/-0.10, LVESD 5.3+/-0.09, p=0.026, p=0.003). There was also evidence of systolic dysfunction in the CS-exposed group compared to the CON group (fractional shortening %, CON=43+/-2, CS=36+/-.09, p=0.010). In CS-exposed hearts, significant increases in phosphorylated p38/total p38 (0.975+/-0.05) and phosphorylated ERK1/2/totalERK1/2 (1.919+/-0.050) were found compared to CON hearts (0.464+/-0.008, 0.459+/-0.050, respectively). No significant differences were found in JNK levels between the groups.
Increased NE levels and MAPK activation are associated with CS-related left ventricular remodelling.
确定接触香烟烟雾（CS）对丝裂原活化蛋白激酶（MAPK）（细胞外信号调节激酶[ERK1/2]、p38激酶[p38]和c-Jun氨基末端蛋白激酶[JNK]）的表达/激活、去甲肾上腺素（NE）水平以及心肌结构和功能的影响。
将大鼠随机分为两组：接触CS组（n = 12）和室内空气对照组（CON）（n = 10）。5周后，对动物进行超声心动图检查并测量脉搏波多普勒血流。取出心脏进行显微镜检查和蛋白质印迹分析。
接触CS与NE尿水平显著升高以及心室尺寸增大有关（毫米）（CON = 左心室舒张末期内径[LVEDD] 7.99±0.10，左心室收缩末期内径[LVESD] 4.55±0.20，CS = LVEDD 8.3±0.10，LVESD 5.3±0.09，p = 0.026，p = 0.003）。与CON组相比，接触CS组也有收缩功能障碍的证据（缩短分数%，CON = 43±2，CS = 36±0.09，p = 0.010）。与CON组心脏相比，在接触CS的心脏中，发现磷酸化p38/总p38（0.975±0.05）和磷酸化ERK1/2/总ERK1/2（1.919±0.050）显著增加（分别为0.464±0.008和0.459±0.050）。两组之间JNK水平无显著差异。
NE水平升高和MAPK激活与CS相关的左心室重构有关。
