来自狭叶海桐变种狭叶海桐的苯酞类化合物对中性粒细胞超氧化物生成和弹性蛋白酶释放具有抑制活性。

Phthalides from Pittosporum illicioides var. illicioides with inhibitory activity on superoxide generation and elastase release by neutrophils.

作者信息

Chou Tsung-Hsien, Chen Ih-Sheng, Hwang Tsong-Long, Wang Tai-Chi, Lee Tzong-Huei, Cheng Lin-Yang, Chang Ya-Chih, Cho Jui-Ying, Chen Jih-Jung

机构信息

Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China.

出版信息

J Nat Prod. 2008 Oct;71(10):1692-5. doi: 10.1021/np8004503. Epub 2008 Sep 26.

DOI:10.1021/np8004503

PMID:18817445

Abstract

Six new phthalides, (S)-3-ethyl-7-hydroxy-6-methoxyphthalide (1), (S)-3-ethyl-7-hydroxy-5,6-dimethoxyphthalide (2), (S)-3-ethyl-5,6,7-trimethoxyphthalide (3), (R)-3-ethyl-7-hydroxy-6-methoxyphthalide (4), (Z)-3-ethylidene-7-hydroxy-6-methoxyphthalide (5), and (Z)-3-ethylidene-6,7-dimethoxyphthalide (6), have been isolated from the root of Pittosporum illicioides var. illicioides, together with seven known compounds. The structures of these new compounds were determined through spectroscopic and MS analyses. Compounds 1-4 exhibited inhibition (IC50<or=29.8 microM) of superoxide anion generation by human neutrophils in response to formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B (fMLP/CB). Compounds 5 and 6 inhibited fMLP/CB-induced elastase release with IC50 values of 38.6+/-4.3 and 33.9+/-3.9 microM, respectively.

摘要

从少花海桐根中分离得到6个新的苯酞类化合物，即(S)-3-乙基-7-羟基-6-甲氧基苯酞(1)、(S)-3-乙基-7-羟基-5,6-二甲氧基苯酞(2)、(S)-3-乙基-5,6,7-三甲氧基苯酞(3)、(R)-3-乙基-7-羟基-6-甲氧基苯酞(4)、(Z)-3-亚乙基-7-羟基-6-甲氧基苯酞(5)和(Z)-3-亚乙基-6,7-二甲氧基苯酞(6)，以及7个已知化合物。通过光谱和质谱分析确定了这些新化合物的结构。化合物1-4对人中性粒细胞在佛波醇酯/细胞松弛素B(fMLP/CB)刺激下产生超氧阴离子具有抑制作用(IC50≤29.8 μM)。化合物5和6对fMLP/CB诱导的弹性蛋白酶释放具有抑制作用，IC50值分别为38.6±4.3和33.9±3.9 μM。