Patel Minal, Vandevord Pamela J, Matthew Howard W, Desilva Stephen, Wu Bin, Wooley Paul H
Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, USA.
Tissue Eng Part C Methods. 2008 Dec;14(4):365-70. doi: 10.1089/ten.tec.2008.0166.
The objective of this work was to use a functional gait analysis technique to evaluate sciatic nerve repair through tissue-engineered nerve guides in a rodent animal model. The nerve guides were fabricated by blending collagen with chitosan material and evaluated over a 12-week period for motor and sensory nerve recovery assessed by gait analysis and behavioral testing. Gastrocnemius muscle weight measurements were obtained at the end of each experimental time point and correlated to motor nerve recovery. Functional gait analysis studied both the stance and swing phase angle formations during a normal gait cycle. During the stance phase, functional results revealed that blended nerve guides promoted increased motor nerve recovery than unblended chitosan nerve guides. Similar results were obtained from behavioral tests, indicating that blended nerve guides created increased sensitivity to applied stimulus compared to unblended nerve guides. Muscle strength also correlated with functional recovery and was significantly higher when compared to the unblended nerve guides. From this study, we conclude that collagen-blended chitosan nerve guides enhanced motor and sensory nerve recovery assayed through gait and behavioral testing compared to unblended nerve guides.
这项工作的目的是在啮齿动物模型中，使用功能步态分析技术通过组织工程神经导管评估坐骨神经修复情况。这些神经导管是通过将胶原蛋白与壳聚糖材料混合制成的，并在12周的时间内进行评估，通过步态分析和行为测试来评估运动和感觉神经的恢复情况。在每个实验时间点结束时测量腓肠肌重量，并将其与运动神经恢复情况相关联。功能步态分析研究了正常步态周期中的站立期和摆动期角度形成。在站立期，功能结果显示，与未混合的壳聚糖神经导管相比，混合神经导管促进了更多的运动神经恢复。行为测试也得到了类似的结果，表明与未混合的神经导管相比，混合神经导管对施加的刺激产生了更高的敏感性。肌肉力量也与功能恢复相关，与未混合的神经导管相比明显更高。从这项研究中，我们得出结论，与未混合的神经导管相比，胶原蛋白混合壳聚糖神经导管通过步态和行为测试增强了运动和感觉神经的恢复。
