Patel Minal, Vandevord Pamela J, Matthew Howard, Wu Bin, DeSilva Stephen, Wooley Paul H
Department of Biomedical Engineering, Wayne State University School of Medicine, Detroit, Michigan, USA.
Tissue Eng. 2006 Nov;12(11):3189-99. doi: 10.1089/ten.2006.12.3189.
Quantitative analysis of peripheral nerve regeneration using nerve guides is commonly evaluated through histomorphometry and walking track analysis. We conducted a unique assessment of functional sciatic nerve recovery treated with chitosan nerve guides. We used video-gait analysis to evaluate the extent of functional nerve recovery by measuring the ankle angle at different gait cycle phases. We also correlated the gastrocnemius muscle weight measurements and histological analysis to functional nerve recovery. The chitosan group showed increased functional improvement compared to the control groups at the end of a 12-week period ( p < 0.05). Although both control and chitosan angle measurements were lower than those recorded for presurgery animals, the angle measurements significantly improved over the 12-week period. Stance phase duration of the gait cycle was also recorded, which showed a significant increase over the 12-week time period. The muscle weight parameter indicated a significant decrease in muscle atrophy and restoration of functional strength. Histological analysis revealed that the chitosan nerve guide provided significantly increased axonal growth. The functional results indicated that chitosan nerve guides enhanced functional improvement over no repair processes.
使用神经导管对周围神经再生进行定量分析通常通过组织形态计量学和行走轨迹分析来评估。我们对用壳聚糖神经导管治疗的坐骨神经功能恢复进行了独特的评估。我们使用视频步态分析，通过测量不同步态周期阶段的踝关节角度来评估神经功能恢复的程度。我们还将腓肠肌重量测量和组织学分析与神经功能恢复相关联。在12周结束时，壳聚糖组与对照组相比功能改善有所增加（p < 0.05）。尽管对照组和壳聚糖组的角度测量值均低于术前动物记录的值，但在12周期间角度测量值有显著改善。还记录了步态周期的站立期持续时间，其在12周时间段内显著增加。肌肉重量参数表明肌肉萎缩显著减少且功能强度得以恢复。组织学分析显示壳聚糖神经导管使轴突生长显著增加。功能结果表明，与未进行修复的情况相比，壳聚糖神经导管增强了功能改善。
