Sundararajan Raji
ECET Department, Purdue University, W. Lafayette, IN 47907, USA.
Mol Biotechnol. 2009 Jan;41(1):69-82. doi: 10.1007/s12033-008-9107-y. Epub 2008 Sep 26.
As the medical field moves from treatment of diseases with drugs to treatment with genes, safe and efficient gene delivery systems are needed to make this transition. One such safe, non-viral, and efficient gene delivery system is electroporation (electrogenetherapy). Exciting discoveries using electroporation could make this technique applicable to drug and vaccine delivery in addition to gene delivery. Typically milli and microsecond pulses have been used for electroporation. Recently, the use of nanosecond electrical pulses (10-300 ns) at very high magnitudes (10-300 kV/cm) has been studied for direct DNA transfer to the nucleus in vitro. This article reviews the work done using high-intensity nanosecond pulses, termed as nanosecond electroporation (nsEP), in electroporation gene delivery systems.
随着医学领域从药物治疗疾病转向基因治疗，需要安全有效的基因递送系统来实现这一转变。一种这样安全、非病毒且高效的基因递送系统是电穿孔（电基因治疗）。使用电穿孔的激动人心的发现可能使该技术除了基因递送外还适用于药物和疫苗递送。通常，毫微秒和微秒脉冲已用于电穿孔。最近，已研究使用极高强度（10 - 300 kV/cm）的纳秒电脉冲（10 - 300 ns）在体外将DNA直接转移到细胞核。本文综述了在电穿孔基因递送系统中使用高强度纳秒脉冲（称为纳秒电穿孔，nsEP）所做的工作。
