用于通过纳秒脉冲电场对哺乳动物细胞进行非侵入性扰动的电极微腔。

Electrode microchamber for noninvasive perturbation of mammalian cells with nanosecond pulsed electric fields.

作者信息

Sun Yinghua, Vernier P Thomas, Behrend Matthew, Marcu Laura, Gundersen Martin A

机构信息

Materials Science Department, University of Southern California, Los Angeles, CA 90089, USA.

出版信息

IEEE Trans Nanobioscience. 2005 Dec;4(4):277-83. doi: 10.1109/tnb.2005.859544.

DOI:10.1109/tnb.2005.859544

PMID:16433293

Abstract

Nanosecond pulsed electric fields can pass through the external membrane of biological cells and disturb fast-responding intracellular structures and processes. To enable real-time imaging and investigation of these phenomena, a microchamber with integral electrodes and optical path for observing individual cells exposed to ultrashort electric pulses was designed and fabricated utilizing photolithographic and microelectronic methods. SU-8 photoresist was patterned to form straight sidewalls from 10 to 30 microm in height, with gold film deposited on the top and sidewalls for conductive, nonreactive electrodes and a uniform electric field. Channel dimensions (10-30 microm x 100 microm x 12 000 microm) are suitable for observations of mammalian cells during nanosecond, megavolt-per-meter pulsed electric field exposure. Experimental studies utilizing the electrode microchamber include live-cell imaging of nanoelectropulse-induced intracellular calcium bursts and membrane phospholipid translocation.

摘要

纳秒脉冲电场可以穿透生物细胞的外膜，干扰快速响应的细胞内结构和过程。为了能够实时成像和研究这些现象，利用光刻和微电子方法设计并制造了一种带有集成电极和光路的微腔，用于观察暴露于超短电脉冲的单个细胞。SU-8光刻胶被图案化以形成高度为10至30微米的直侧壁，顶部和侧壁上沉积有金膜，用于形成导电、无反应的电极和均匀电场。通道尺寸（10 - 30微米×100微米×12000微米）适合在纳秒、兆伏/米脉冲电场暴露期间观察哺乳动物细胞。利用电极微腔进行的实验研究包括对纳米电脉冲诱导的细胞内钙爆发和膜磷脂转位的活细胞成像。