50赫兹2.45毫特斯拉磁场对顺铂、丝裂霉素C和甲氨蝶呤在酿酒酵母中的效力无影响。

No effect of 50 Hz 2.45 mT magnetic field on the potency of cisplatin, mitomycin C, and methotrexate in S. cerevisiae.

作者信息

Ruiz-Gómez M J, Merino-Moyano M D, Cebrián-Martín M G, Prieto-Barcia M I, Martínez-Morillo M

机构信息

Laboratory of Radiobiology, Department of Radiology and Physical Medicine, Faculty of Medicine, University of Malaga, Malaga, Spain.

出版信息

Electromagn Biol Med. 2008;27(3):289-97. doi: 10.1080/15368370802277740.

DOI:10.1080/15368370802277740

PMID:18821204

Abstract

Drug resistance is an obstacle for chemotherapy success. Because of this, this work aims to improve the cell killing effect of antineoplastic drugs by magnetic field (MF) co-exposure. S. cerevisiae cells were exposed to 2.45 mT, sinusoidal 50 Hz MF, during 48 h, and the drugs cisplatin (cisPt), mitomycin C (MMC), or methotrexate (MTX); 100 and 1,000 microg/ml. Survival was assayed by the drop test. The results showed that MF exposures do not induce alterations in the potency of cisPt, MMC, and MTX on these cells in relation to untreated controls. In addition, a strong correlation between temperature and potency of cisPt was found, which contribute to the establishment of the importance of an exhaustive control of temperature in experiments carried out with temperature sensitive antineoplastic agents in co-exposure with MF; avoiding differences between MF-exposed samples and unexposed controls and contributing to the performance of experiments under well-defined and controlled conditions.

摘要

耐药性是化疗成功的一个障碍。因此，本研究旨在通过联合暴露于磁场（MF）来提高抗肿瘤药物的细胞杀伤效果。将酿酒酵母细胞暴露于2.45 mT、50 Hz正弦MF下48小时，并分别加入顺铂（cisPt）、丝裂霉素C（MMC）或甲氨蝶呤（MTX），浓度分别为100和1000μg/ml。通过点滴试验测定细胞存活率。结果表明，与未处理的对照相比，暴露于MF不会导致cisPt、MMC和MTX对这些细胞的效力发生改变。此外，还发现温度与cisPt的效力之间存在很强的相关性，这有助于确立在与MF联合暴露的温度敏感抗肿瘤药物实验中，全面控制温度的重要性；避免MF暴露样品与未暴露对照之间的差异，并有助于在明确和可控的条件下进行实验。