Jaganathan Hamsa, Kinsella Joseph M, Ivanisevic Albena
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Chemphyschem. 2008 Oct 24;9(15):2203-6. doi: 10.1002/cphc.200800509.
In order to control the fabrication method, the mechanism used in the formation of DNA templated nanowires is investigated through circular dichroism (CD) spectroscopy. Metallic (Au) and magnetic (Fe(2)O(3) and CoFe(2)O(4)) nanoparticles (NP) are aligned along the DNA strand at various mass ratios. The DNA templated nanowires are compared to the structure of B-form dsDNA through CD experiments. Absorbance and thermal melting tests are performed to verify the structural changes of DNA templated nanowires. Low concentrations of nanoparticles preserve the DNA B-form through electrostatic interactions. Conversely, at higher concentrations of nanoparticles aligned along the DNA strand, the template is denatured. Information on the mode of nanoparticle binding and DNA helix alterations are explored for metallic and magnetic nanowires based upon the results.
为了控制制备方法，通过圆二色光谱（CD）研究了DNA模板纳米线形成过程中所使用的机制。金属（Au）和磁性（Fe₂O₃和CoFe₂O₄）纳米颗粒（NP）以各种质量比沿着DNA链排列。通过CD实验将DNA模板纳米线与B型双链DNA的结构进行比较。进行吸光度和热变性测试以验证DNA模板纳米线的结构变化。低浓度的纳米颗粒通过静电相互作用保持DNA的B型结构。相反，当沿着DNA链排列的纳米颗粒浓度较高时，模板会变性。基于这些结果，探索了金属和磁性纳米线的纳米颗粒结合模式及DNA螺旋变化的相关信息。
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