Herrera-Herrera Mireya L, Salazar-Olivo Luis A
División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, San Luis Potosí, México.
Biochem Biophys Res Commun. 2008 Dec 5;377(1):29-34. doi: 10.1016/j.bbrc.2008.09.071. Epub 2008 Sep 25.
RFamide neuropeptides NPFF and NPAF affect gene expression in mature 3T3-L1 adipocytes but their role on adipogenesis is unknown. Here, we show that NPFF, NPAF, and NPSF inhibited the differentiation of 3T3-F442A preadipocytes in a concentration-dependent manner, but had no effect on 3T3-L1 adipogenesis. All three neuropeptides also blocked the adipose differentiation of normal and lipoma-derived human preadipocytes. The antiadipogenic effect of RFamide neuropeptides was linked with the overexpression of Id3 gene and the inhibition by NPAF remained after neuropeptide removal and further incubation of 3T3 cells with adipogenic medium. Our results show that NPFF, NPAF and NPSF negatively affect adipogenesis and suggest that these compounds participate in the regulation of the adipose tissue development by the central nervous system.
RFamide神经肽NPFF和NPAF影响成熟3T3-L1脂肪细胞中的基因表达，但其在脂肪生成中的作用尚不清楚。在此，我们表明NPFF、NPAF和NPSF以浓度依赖的方式抑制3T3-F442A前脂肪细胞的分化，但对3T3-L1脂肪生成没有影响。这三种神经肽还阻断了正常和脂肪瘤来源的人前脂肪细胞的脂肪分化。RFamide神经肽的抗脂肪生成作用与Id3基因的过表达有关，并且在去除神经肽并将3T3细胞与成脂培养基进一步孵育后，NPAF的抑制作用仍然存在。我们的结果表明，NPFF、NPAF和NPSF对脂肪生成有负面影响，并表明这些化合物参与中枢神经系统对脂肪组织发育的调节。
