恶性疟原虫：青蒿素对在镰状性状血红蛋白AS和正常血红蛋白AA红细胞中培养的恶性疟原虫的活性。

Plasmodium falciparum: Activity of artemisinin against Plasmodium falciparum cultured in sickle trait hemoglobin AS and normal hemoglobin AA red blood cells.

作者信息

Walcourt A, Kurantsin-Mills J, Adenuga B B, Loyevsky M, Kassim O O, Gordeuk V R

机构信息

Department of Physiology & Biophysics, Howard University College of Medicine, 520 W Street, Seely G Mudd Building, NW, Washington, DC 20059, USA.

出版信息

Exp Parasitol. 2008 Dec;120(4):381-4. doi: 10.1016/j.exppara.2008.09.006. Epub 2008 Sep 13.

DOI:10.1016/j.exppara.2008.09.006

PMID:18823976

Abstract

The presence of sickle hemoglobin causes accumulation of hemoglobin degradative products that favor oxidative reaction in erythrocytes. Artemisinin derivatives exert antiparasite effects through oxidative reactions within infected erythrocytes. Using [(3)H]-hypoxanthine incorporation, we therefore did an in vitro comparison of IC(50) values for artemisinin in Plasmodium falciparum-infected erythrocytes from sickle cell trait (AS) and normal (AA) individuals. IC(50) values for chloroquine served as control. Without drugs, parasite growth was similar in AA and AS erythrocytes. Gender, age and blood group of donors had no significant effects on parasite growth. IC(50) value for artemisinin was 27+/-14nM in AS (N=22) compared to 24+/-9nM (N=27) in AA erythrocytes (P=0.4). IC(50) values for chloroquine were also similar in AA (22+/-8nM) and AS (20+/-11nM) erythrocytes. These results show no evidence of elevated artemisinin activity on P. falciparum in AS erythrocytes in vitro.

摘要

镰状血红蛋白的存在会导致血红蛋白降解产物的积累，这些产物有利于红细胞内的氧化反应。青蒿素衍生物通过感染红细胞内的氧化反应发挥抗寄生虫作用。因此，我们利用[³H] - 次黄嘌呤掺入法，对镰状细胞性状（AS）个体和正常（AA）个体的恶性疟原虫感染红细胞中青蒿素的半数抑制浓度（IC₅₀）值进行了体外比较。氯喹的IC₅₀值作为对照。在无药物情况下，AA和AS红细胞中的寄生虫生长情况相似。供体的性别、年龄和血型对寄生虫生长无显著影响。AS组（N = 22）青蒿素的IC₅₀值为27±14nM，而AA红细胞组为24±9nM（N = 27）（P = 0.4）。氯喹在AA（22±8nM）和AS（20±11nM）红细胞中的IC₅₀值也相似。这些结果表明，体外实验中没有证据显示青蒿素对AS红细胞中的恶性疟原虫活性增强。