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本文引用的文献

1
RNA recognition motifs: boring? Not quite.RNA识别基序：乏味？并非如此。
Curr Opin Struct Biol. 2008 Jun;18(3):290-8. doi: 10.1016/j.sbi.2008.04.002.
2
Soluble expanded PABPN1 promotes cell death in oculopharyngeal muscular dystrophy.可溶性扩展型PABPN1促进眼咽型肌营养不良中的细胞死亡。
Neurobiol Dis. 2007 Jun;26(3):546-57. doi: 10.1016/j.nbd.2007.02.004. Epub 2007 Feb 15.
3
Three-dimensional structure determined for a subunit of human tRNA splicing endonuclease (Sen15) reveals a novel dimeric fold.已确定的人tRNA剪接内切核酸酶（Sen15）一个亚基的三维结构揭示了一种新型二聚体折叠。
J Mol Biol. 2007 Feb 9;366(1):155-64. doi: 10.1016/j.jmb.2006.11.024. Epub 2006 Nov 11.
4
A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1.眼咽型肌营养不良的果蝇模型揭示了聚腺苷酸结合蛋白核 1 的内在毒性。
EMBO J. 2006 May 17;25(10):2253-62. doi: 10.1038/sj.emboj.7601117. Epub 2006 Apr 27.
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High-throughput purification and quality assurance of Arabidopsis thaliana proteins for eukaryotic structural genomics.用于真核生物结构基因组学的拟南芥蛋白质的高通量纯化与质量保证。
J Struct Funct Genomics. 2005;6(2-3):143-7. doi: 10.1007/s10969-005-1908-7.
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Archived gels as a tool for identification of protein complexes: Polo kinase cofractionates with Drosophila 205-kDa MAP and ncd in mitotic embryonic extracts.
Anal Biochem. 2005 Sep 1;344(1):155-7. doi: 10.1016/j.ab.2005.06.023.
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The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression.RNA识别基序，一种调控转录后基因表达的可塑性RNA结合平台。
FEBS J. 2005 May;272(9):2118-31. doi: 10.1111/j.1742-4658.2005.04653.x.
8
Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination.用于生产用于核磁共振筛选和结构测定的[U-15N]标记和[U-13C, U-15N]标记蛋白质的自诱导培养基。
Protein Expr Purif. 2005 Apr;40(2):268-78. doi: 10.1016/j.pep.2004.12.024.
9
Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination.利用位点特异性重组对拟南芥靶基因进行高通量DNA克隆的结果。
J Struct Funct Genomics. 2004;5(4):267-76. doi: 10.1007/s10969-004-7148-4. Epub 2005 Mar 2.
10
Identification of a novel Xenopus laevis poly (A) binding protein.
Biol Cell. 2004 Sep;96(7):519-27. doi: 10.1016/j.biolcel.2004.04.006.

II型聚腺苷酸结合蛋白识别RNA的结构基础。

Structural basis for RNA recognition by a type II poly(A)-binding protein.

作者信息

Song Jikui, McGivern Jered V, Nichols Karl W, Markley John L, Sheets Michael D

机构信息

Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin, Madison, WI 53706, USA.

出版信息

Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15317-22. doi: 10.1073/pnas.0801274105. Epub 2008 Sep 29.

DOI:10.1073/pnas.0801274105
PMID:18824697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2563106/
Abstract

We identified a functional domain (XlePABP2-TRP) of Xenopus laevis embryonic type II poly(A)-binding protein (XlePABP2). The NMR structure of XlePABP2-TRP revealed that the protein is a homodimer formed by the antiparallel association of beta-strands from the single RNA recognition motif (RRM) domain of each subunit. In each subunit of the homodimer, the canonical RNA recognition site is occluded by a polyproline motif. Upon poly(A) binding, XlePABP2-TRP undergoes a dimer-monomer transition that removes the polyproline motif from the RNA recognition site and allows it to be replaced by the adenosine nucleotides of poly(A). Our results provide high-resolution structural information concerning type II PABPs and an example of a single RRM domain protein that transitions from a homodimer to a monomer upon RNA binding. These findings advance our understanding of RRM domain regulation, poly(A) recognition, and are relevant to understanding how type II PABPs function in mRNA processing and human disease.

摘要

我们鉴定出了非洲爪蟾胚胎II型聚腺苷酸结合蛋白（XlePABP2）的一个功能结构域（XlePABP2-TRP）。XlePABP2-TRP的核磁共振结构显示，该蛋白是一个同型二聚体，由每个亚基的单个RNA识别基序（RRM）结构域中的β链反平行缔合形成。在同型二聚体的每个亚基中，典型的RNA识别位点被一个多聚脯氨酸基序所遮蔽。在结合聚腺苷酸后，XlePABP2-TRP会发生二聚体-单体转变，将多聚脯氨酸基序从RNA识别位点移除，并使其被聚腺苷酸的腺苷核苷酸取代。我们的结果提供了关于II型聚腺苷酸结合蛋白的高分辨率结构信息，以及一个单一RRM结构域蛋白在结合RNA后从同型二聚体转变为单体的例子。这些发现推进了我们对RRM结构域调控、聚腺苷酸识别的理解，并且与理解II型聚腺苷酸结合蛋白在mRNA加工和人类疾病中的功能相关。