Asadullaev T A, Shekhtman A B, Lurie L M, Bukrinskaia A G
Vopr Virusol. 1976 Jul-Aug(4):403-10.
Hybridization of Ehrlich ascitic carcinoma cells and chicken fibroblasts using UV-inactivated Sendai virus produced the following variants of homo- and heterokaryons: (a) uninfected Ehrlich cells, (b) fowl plague virus-infected chicken fibroblasts, (c) uninfected Ehrlich cells and uninfected chicken fibroblasts; (d) infected Ehrlich cells and infected chicken fibroblasts, (e) infected Ehrlich cells and uninfected chicken fibroblasts. The analysis of the material produced by heterokaryons gave the following results: when Ehrlich ascitic carcinoma cells infected with classical fowl plaque virus and labeled with 3H-uridine fused with uninfected unlabeled chicken fibroblasts, the heterokaryons, in addition to structures with the buoyant density of 1.29 g/ml typical for infected cells, also produced virus structures with a density of 1.25 g/ml and had a higher infectious activity (5.2 X 10(7) PFU/ml) than the structures produced by infected Ehrlich cells (5.8 X 10(6) PFU/ml); after fusion of Ehrlich ascitic carcinoma cells infected with fowl plague virus and labelled with 3H-uridine with unlabelled chicken fibroblasts infected with fowl plague virus heterokaryons produced virus structures with densities of 1.29 g/ml and 1.22 g/ml which had even higher infectious activity (8.6 X 10(8) PFU/ml) than the structures produced by infected Ehlrich ascitic cells.
使用紫外线灭活的仙台病毒使艾氏腹水癌细胞与鸡成纤维细胞杂交，产生了以下同核体和异核体变体：(a) 未感染的艾氏细胞，(b) 感染禽痘病毒的鸡成纤维细胞，(c) 未感染的艾氏细胞和未感染的鸡成纤维细胞；(d) 感染的艾氏细胞和感染的鸡成纤维细胞，(e) 感染的艾氏细胞和未感染的鸡成纤维细胞。对异核体产生的物质进行分析得到以下结果：当用经典禽痘病毒感染并用3H-尿苷标记的艾氏腹水癌细胞与未感染的未标记鸡成纤维细胞融合时，异核体除了产生感染细胞典型的浮力密度为1.29 g/ml的结构外，还产生了密度为1.25 g/ml的病毒结构，并且具有比感染的艾氏细胞产生的结构更高的感染活性（5.2×10(7) PFU/ml）（5.8×10(6) PFU/ml）；在用禽痘病毒感染并用3H-尿苷标记的艾氏腹水癌细胞与感染禽痘病毒的未标记鸡成纤维细胞融合后，异核体产生了密度为1.29 g/ml和1.22 g/ml的病毒结构，其感染活性（8.6×10(8) PFU/ml）甚至比感染的艾氏腹水细胞产生的结构更高。
