从猪葡萄球菌猪亚种中分离剥脱毒素及其在仔猪中的剥脱活性。

Isolation of exfoliative toxin from Staphylococcus hyicus subsp. hyicus and its exfoliative activity in the piglet.

作者信息

Sato H, Tanabe T, Kuramoto M, Tanaka K, Hashimoto T, Saito H

机构信息

Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.

出版信息

Vet Microbiol. 1991 May;27(3-4):263-75. doi: 10.1016/0378-1135(91)90153-7.

DOI:10.1016/0378-1135(91)90153-7

PMID:1882504

Abstract

Exfoliative toxin was isolated from the sterile cell-free filtrate of 24 h culture of Staphylococcus hyicus subsp. hyicus strain P-1. The partial purification of exfoliative toxin produced by S. hyicus (shET) was performed by precipitation with 50-80% saturated ammonium sulfate, gel filtration on a Sephadex G-75 column and column chromatography on DEAE-cellulose. Partially purified shET (pp-shET) caused exfoliation in piglets at 8 to 12 h after intradermal or subcutaneous injection. However, heat-treated pp-shET did not cause exfoliation in piglets for up to 24 h after injection. On histopathological examination of the skin at 12 h after injection of pp-shET, an intraepidermal cleavage plane was shown between the stratum corneum and stratum granulosum and at the stratum granulosum.

摘要

从猪葡萄球菌亚种猪葡萄球菌P-1菌株24小时培养物的无菌无细胞滤液中分离出剥脱毒素。通过用50-80%饱和硫酸铵沉淀、在Sephadex G-75柱上进行凝胶过滤以及在DEAE-纤维素上进行柱色谱法对猪葡萄球菌产生的剥脱毒素（shET）进行部分纯化。部分纯化的shET（pp-shET）在皮内或皮下注射后8至12小时在仔猪中引起表皮脱落。然而，经热处理的pp-shET在注射后长达24小时内未在仔猪中引起表皮脱落。在注射pp-shET后12小时对皮肤进行组织病理学检查时，在角质层和颗粒层之间以及颗粒层处显示出表皮内分裂平面。