Andresen L O
Department of Microbiology, Danish Veterinary Laboratory, Copenhagen V.
FEMS Immunol Med Microbiol. 1998 Apr;20(4):301-10. doi: 10.1111/j.1574-695X.1998.tb01140.x.
Exfoliative toxins of approximately 30 kDa produced by Staphylococcus hyicus strains NCTC 10350, 1289D-88 and 842A-88 were purified and specific polyclonal antisera were raised against each of the toxins. It was shown by immunoblot analysis and ELISA that three exfoliative toxins from S. hyicus were antigenically distinct. The three toxins were designated ExhA, ExhB and ExhC. From 60 diseased pigs, each representing an outbreak of exudative epidermitis, a total of 584 isolates of S. hyicus were phage typed and tested for production of exfoliative toxin. ExhA-, ExhB- and ExhC-producing S. hyicus isolates were found in 12 (20%), 20 (33%) and 11 (18%), respectively, of the 60 pig herds investigated. Production of the different types of exfoliative toxin was predominantly associated with certain phage groups. However, toxin production was found in all of the six phage groups defined by the phage typing system. Some changes in the distribution of isolates between phage groups were observed when the results of this study were compared to previous investigations. In this study two new antigenically distinct exfoliative toxins were isolated and tools for in vitro detection of toxin producing S. hyicus isolates and for further studies on the exfoliative toxins from S. hyicus have been provided.
对来自猪葡萄球菌菌株NCTC 10350、1289D - 88和842A - 88产生的约30 kDa的剥脱毒素进行了纯化，并针对每种毒素制备了特异性多克隆抗血清。免疫印迹分析和酶联免疫吸附测定表明，来自猪葡萄球菌的三种剥脱毒素在抗原性上是不同的。这三种毒素分别命名为ExhA、ExhB和ExhC。从60头患有渗出性皮炎暴发的患病猪中，共分离出584株猪葡萄球菌，并进行噬菌体分型和剥脱毒素产生检测。在所调查的60个猪群中，分别有12个（20%）、20个（33%）和11个（18%）猪群分离出产生ExhA、ExhB和ExhC的猪葡萄球菌菌株。不同类型剥脱毒素的产生主要与某些噬菌体组相关。然而，在所定义的噬菌体分型系统的所有六个噬菌体组中均发现了毒素产生。当将本研究结果与先前调查结果进行比较时，观察到噬菌体组之间分离株分布的一些变化。在本研究中，分离出了两种新的抗原性不同的剥脱毒素，并提供了用于体外检测产毒素猪葡萄球菌分离株以及进一步研究猪葡萄球菌剥脱毒素的工具。
