Mysiakina I S, Funtikova N S
Mikrobiologiia. 2008 Jul-Aug;77(4):460-4.
It is shown that the fungus Mucor circinelloides var. lusitanicus INMI grown under aerobic conditions in a medium with a high glucose concentration (20%) is capable of both yeastlike and mycelial growth. In the mycelium, the activity of NAD-dependent isocitrate dehydrogenase was more than twice as high as in yeastlike cells, whereas the isocitrate lyase activity was lower. A number of significant differences were found in the lipid composition of the cells of two different morphological variants. Yeastlike cells contained more polar lipids and free fatty acids and less principal reserve lipids (triacylglycerides) than mycelial cells; the content of gamma-linolenic acid and the degree of lipid unsaturation were significantly lower in these cells than in the mycelium. In yeastlike cells, glycolipids composed the bulk of polar lipids; the proportion of phospholipids (primarily phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, and cardiolipin) was lower. The relationship between cellular metabolism and the lipid composition of fungal cells of different morphotypes grown at high concentrations of glucose, one of the main inducers of dimorphic growth, is discussed.
结果表明，在有氧条件下于含高浓度葡萄糖（20%）的培养基中生长的葡枝根霉INMI变种真菌，能够进行酵母样生长和菌丝体生长。在菌丝体中，NAD依赖的异柠檬酸脱氢酶活性比酵母样细胞中的活性高出两倍多，而异柠檬酸裂解酶活性则较低。在两种不同形态变体的细胞脂质组成中发现了许多显著差异。酵母样细胞比菌丝体细胞含有更多的极性脂质和游离脂肪酸，而主要储备脂质（三酰甘油）较少；这些细胞中的γ-亚麻酸含量和脂质不饱和度程度明显低于菌丝体。在酵母样细胞中，糖脂构成了极性脂质的主体；磷脂（主要是磷脂酰丝氨酸、磷脂酰胆碱、磷脂酰乙醇胺和心磷脂）的比例较低。本文讨论了细胞代谢与在高浓度葡萄糖（双态生长的主要诱导剂之一）条件下生长的不同形态型真菌细胞脂质组成之间的关系。
