Gross Roy, Guzman Carlos A, Sebaihia Mohammed, dos Santos Vítor A P Martins, Pieper Dietmar H, Koebnik Ralf, Lechner Melanie, Bartels Daniela, Buhrmester Jens, Choudhuri Jomuna V, Ebensen Thomas, Gaigalat Lars, Herrmann Stefanie, Khachane Amit N, Larisch Christof, Link Stefanie, Linke Burkhard, Meyer Folker, Mormann Sascha, Nakunst Diana, Rückert Christian, Schneiker-Bekel Susanne, Schulze Kai, Vorhölter Frank-Jörg, Yevsa Tetyana, Engle Jacquelyn T, Goldman William E, Pühler Alfred, Göbel Ulf B, Goesmann Alexander, Blöcker Helmut, Kaiser Olaf, Martinez-Arias Rosa
Chair of Microbiology, Biocenter, University of Würzburg, Am Hubland, D-97074 Würzburg, Germany.
BMC Genomics. 2008 Sep 30;9:449. doi: 10.1186/1471-2164-9-449.
Bordetella petrii is the only environmental species hitherto found among the otherwise host-restricted and pathogenic members of the genus Bordetella. Phylogenetically, it connects the pathogenic Bordetellae and environmental bacteria of the genera Achromobacter and Alcaligenes, which are opportunistic pathogens. B. petrii strains have been isolated from very different environmental niches, including river sediment, polluted soil, marine sponges and a grass root. Recently, clinical isolates associated with bone degenerative disease or cystic fibrosis have also been described.
In this manuscript we present the results of the analysis of the completely annotated genome sequence of the B. petrii strain DSMZ12804. B. petrii has a mosaic genome of 5,287,950 bp harboring numerous mobile genetic elements, including seven large genomic islands. Four of them are highly related to the clc element of Pseudomonas knackmussii B13, which encodes genes involved in the degradation of aromatics. Though being an environmental isolate, the sequenced B. petrii strain also encodes proteins related to virulence factors of the pathogenic Bordetellae, including the filamentous hemagglutinin, which is a major colonization factor of B. pertussis, and the master virulence regulator BvgAS. However, it lacks all known toxins of the pathogenic Bordetellae.
The genomic analysis suggests that B. petrii represents an evolutionary link between free-living environmental bacteria and the host-restricted obligate pathogenic Bordetellae. Its remarkable metabolic versatility may enable B. petrii to thrive in very different ecological niches.
彼得氏博德特氏菌是迄今在博德特氏菌属中宿主受限的致病成员中发现的唯一环境物种。在系统发育上，它连接了致病的博德特氏菌以及无色杆菌属和产碱杆菌属的环境细菌，这些都是机会致病菌。彼得氏博德特氏菌菌株已从非常不同的环境生态位中分离出来，包括河流沉积物、污染土壤、海棉和草根。最近，也有与骨退行性疾病或囊性纤维化相关的临床分离株的报道。
在本论文中，我们展示了对彼得氏博德特氏菌DSMZ12804菌株完全注释的基因组序列分析结果。彼得氏博德特氏菌有一个5,287,950 bp的镶嵌基因组，含有大量可移动遗传元件，包括七个大的基因组岛。其中四个与克纳氏假单胞菌B13的clc元件高度相关，该元件编码参与芳烃降解的基因。尽管是环境分离株，但测序的彼得氏博德特氏菌菌株也编码与致病博德特氏菌毒力因子相关的蛋白质，包括丝状血凝素，它是百日咳博德特氏菌的主要定植因子，以及主要毒力调节因子BvgAS。然而，它缺乏致病博德特氏菌所有已知的毒素。
基因组分析表明，彼得氏博德特氏菌代表了自由生活的环境细菌与宿主受限的专性致病博德特氏菌之间的进化联系。其显著的代谢多样性可能使彼得氏博德特氏菌能够在非常不同的生态位中茁壮成长。
