Aricò B, Gross R, Smida J, Rappuoli R
Sclavo Research Centre, Siena, Italy.
Mol Microbiol. 1987 Nov;1(3):301-8. doi: 10.1111/j.1365-2958.1987.tb01936.x.
The nucleotide sequence of the pertussis toxin operon of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica, has shown that the last two species contain many common mutations and are likely to derive from a common ancestor (Aricò and Rappuoli, 1987). To elucidate further the evolutionary relationships between the Bordetella species, we have cloned and sequenced the promoter region and the gene coding for the S1 subunit of pertussis toxin from additional B. pertussis strains, such as the type strain BP 18323 and two recent clinical isolates, namely strain BP 13456 from Sweden and strain BP SA1 from Italy. While the strains BP SA1 and BP 13456 are shown to differ from the published B. pertussis sequences by only one base pair, the type strain BP 18323 contains a total of 11 base-pair substitutions. Remarkably, 9 of the 11 substitutions found in BP 18323 are also common to B. parapertussis and B. bronchiseptica, strongly suggesting that this strain derives from the same ancestor as B. parapertussis and B. bronchiseptica. Computer analysis of the sequence data allows the construction of an evolutionary 'tree' showing that the B. pertussis strains are very homogeneous and significantly distant from B. parapertussis and B. bronchiseptica. Therefore the proposed conversion from B. parapertussis to B. pertussis appears highly improbable.
百日咳博德特氏菌、副百日咳博德特氏菌和支气管败血博德特氏菌的百日咳毒素操纵子的核苷酸序列表明，后两个菌种含有许多共同的突变，很可能源自一个共同的祖先（阿里科和拉普奥利，1987年）。为了进一步阐明博德特氏菌各菌种之间的进化关系，我们从其他百日咳博德特氏菌菌株中克隆并测序了启动子区域以及编码百日咳毒素S1亚基的基因，这些菌株包括模式菌株BP 18323以及两株近期的临床分离株，即来自瑞典的菌株BP 13456和来自意大利的菌株BP SA1。虽然菌株BP SA1和BP 13456与已发表的百日咳博德特氏菌序列仅相差一个碱基对，但模式菌株BP 18323总共含有11个碱基对替换。值得注意的是，在BP 18323中发现的11个替换中有9个在副百日咳博德特氏菌和支气管败血博德特氏菌中也很常见，这强烈表明该菌株与副百日咳博德特氏菌和支气管败血博德特氏菌源自同一个祖先。对序列数据的计算机分析使得构建一个进化“树”成为可能，该“树”显示百日咳博德特氏菌菌株非常同质，并且与副百日咳博德特氏菌和支气管败血博德特氏菌有显著差异。因此，从副百日咳博德特氏菌向百日咳博德特氏菌的转变似乎极不可能。
