Jakab Csaba, Halász Judit, Szász Attila Marcell, Batmunkh Enkhjargal, Kiss András, Schaff Zsuzsa, Rusvai Miklós, Gálfi Péter, Kulka Janina
Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, Szent István University, H-1078 Budapest, István u. 2, Hungary.
Acta Vet Hung. 2008 Sep;56(3):341-52. doi: 10.1556/AVet.56.2008.3.8.
The recently identified claudins are dominant components of tight junctions, responsible for cell adhesion, polarity and paracellular permeability. Certain claudins have been shown to have relevance in tumour development. The aim of the present study was to analyse the expression of claudin-1, -2, -3, -4, -5, -7 and -10 in normal canine mammary glands. Samples from the inguinal mammary regions of 20 non-castrated, 1-13 years old female dogs were studied. Immunohistochemical analysis was performed on conventional specimens and tissue microarrays. The results of the immunohistochemical reactions detecting claudins in tissue sections were photodocumented. The immunoreactivity of claudins was quantitatively analysed on digital images using Leica QWin morphometry software. Intense membranous immunolabelling was found for claudin-1, -3 and -7, intense membranous with non-granular cytoplasmic immunolabelling for claudin-2, moderate membranous immunolabelling for claudin-4 and -5, and weak membranous immunolabelling for claudin-10. The occurrence of tight junctions was confirmed by ultrathin section electron microscopy. The available data suggested that claudins might be proteins preserved throughout the evolution of mammals. The results of our study support the concept that they are indeed preserved, since the same type of claudins, in identical distribution, could be detected in our canine mammary tissue samples as could be found in human mammary tissue.
最近发现的紧密连接蛋白是紧密连接的主要成分，负责细胞黏附、极性和细胞旁通透性。已表明某些紧密连接蛋白与肿瘤发展相关。本研究的目的是分析紧密连接蛋白-1、-2、-3、-4、-5、-7和-10在正常犬乳腺中的表达。研究了20只1 - 13岁未绝育雌性犬腹股沟乳腺区域的样本。对常规标本和组织芯片进行了免疫组织化学分析。对组织切片中检测紧密连接蛋白的免疫组织化学反应结果进行了拍照记录。使用徕卡QWin形态测量软件对数字图像上紧密连接蛋白的免疫反应性进行了定量分析。发现紧密连接蛋白-1、-3和-7有强烈的膜免疫标记，紧密连接蛋白-2有强烈的膜及非颗粒状细胞质免疫标记，紧密连接蛋白-4和-5有中等程度的膜免疫标记，紧密连接蛋白-10有微弱的膜免疫标记。通过超薄切片电子显微镜证实了紧密连接的存在。现有数据表明紧密连接蛋白可能是在哺乳动物进化过程中保留下来的蛋白质。我们的研究结果支持这样一种观点，即它们确实是保留下来的，因为在我们的犬乳腺组织样本中可以检测到与人类乳腺组织中相同类型且分布相同的紧密连接蛋白。
